WB
H
Endogenous
250
Rabbit
#Q9Y4D7
23129
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1665 of human PLXND1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
PLXND1 (PlexinD1) is a type I transmembrane receptor for semaphorin (SEMA) family signaling molecules (1). PLXND1 has an extracellular SEMA binding domain, and a cytoplasmic tail containing RasGAP motifs and a RhoGTPase-binding domain. Upon ligand binding, PLXND1 undergoes conformational change and acquires GAP activity that inactivates downstream Rac/Ras signaling, leading to focal adhesion destabilization (2). The PLXND1 signaling pathway plays important roles in neuronal synapse formation, vascular branching, and thymocyte migration (2-4). Increased expression of PLXND1 is positively correlated with tumor stages in multiple cancer types (6). This is supported by gene knockdown experiments that suggest that SEMA/PLXND1 signaling may contribute metastatic progression (7-8).
- Gu, C. and Giraudo, E. (2013) Exp Cell Res 319, 1306-16.
- Gay, C.M. et al. (2011) Dev Biol 349, 1-19.
- Pecho-Vrieseling, E. et al. (2009) Nature 459, 842-6.
- Zygmunt, T. et al. (2011) Dev Cell 21, 301-14.
- Choi, Y.I. et al. (2008) Immunity 29, 888-98.
- Rehman, M. et al. (2016) PLoS One 11, e0164660.
- Tseng, C.H. et al. (2011) PLoS One 6, e19396.
- Casazza, A. et al. (2010) J Clin Invest 120, 2684-98.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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