IHC-P, IF-IC
H
Endogenous
48-200
Rabbit IgG
#P29590
5371
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:500 - 1:2000 |
| Immunofluorescence (Immunocytochemistry) | 1:500 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PML protein.
Background
Promyelocytic leukemia protein (PML) functions via its association with PML bodies in the nucleus (1). PML bodies are dynamic protein aggregates that are interspersed between chromatin in the nuclei of most mammalian cells. The PML protein acts as a scaffold in PML bodies to recruit other proteins, a process regulated by post-translational modifications, such as sumoylation. PML bodies function to regulate a large number of cellular processes, such as tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense (1). The chromosomal translocation t(15;17)(q21;q21) involving the fusion of PML and RARalpha is found in acute promyelocytic leukemia (APL), while a second translocation t(9;15)(p13;q24) involving the fusion of PML and PAX5 was found in acute lymphoblastic leukemia (ALL) (2-6). In addition, PML is frequently inactivated or downregulated in cancer.
- Lallemand-Breitenbach, V. and de Thé, H. (2018) Curr Opin Cell Biol 52, 154-61.
- de Thé, H. et al. (1991) Cell 66, 675-84.
- Goddard, A.D. et al. (1991) Science 254, 1371-4.
- Nebral, K. et al. (2007) Br J Haematol 139, 269-74.
- Qiu, J.J. et al. (2011) Oncogene 30, 967-77.
- Kurahashi, S. et al. (2011) Oncogene 30, 1822-30.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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