Revision 9
#26981
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
IHC-P, FC-FP, FC-L
Reactivity:
H
Sensitivity:
Endogenous
Source/Isotype:
Mouse IgG1
UniProt ID:
#Q86YL7
Entrez-Gene Id:
10630
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:125 - 1:500 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
| Flow Cytometry (Live) | 1:400 - 1:1600 |
Storage
Specificity/Sensitivity
Source / Purification
Background
Podoplanin is O-glycosylated at multiple sites in the extracellular domain, including disialylation at Thr52 in the third platelet-aggregation (PLAG3) domain (10). Research studies have shown that glycoylation at this site is required for tumor cell-induced platelet aggregation activity (11).
Background References
- Breiteneder-Geleff, S. et al. (1997) Am J Pathol 151, 1141-52.
- Breiteneder-Geleff, S. et al. (1999) Am J Pathol 154, 385-94.
- Wicki, A. et al. (2006) Cancer Cell 9, 261-72.
- Omachi, T. et al. (2007) Cancer Lett 246, 167-72.
- Feng, J.Q. et al. (2012) Oral Oncol 48, 848-852.
- Mishima, K. et al. (2006) Acta Neuropathol 111, 563-8.
- Raica, M. et al. Anticancer Res 28, 2997-3006.
- Schoppmann, S.F. et al. (2012) Breast Cancer Res Treat 134, 237-44.
- Ito, M. et al. (2012) Chest 142, 151-158.
- Kato, Y. et al. (2016) PLoS One 11, e0152912.
- Kaneko, M.K. et al. (2007) FEBS Lett 581, 331-6.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IHC-P: Immunohistochemistry (Paraffin) FC-FP: Flow Cytometry (Fixed/Permeabilized) FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
H: Human
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 9
Revision 9
Revision 9
Flow cytometric analysis of live Saos-2 cells (blue, negative) and U-2 0S cells (green, positive) using Podoplanin (LpMab-12) Mouse mAb (solid lines) or a concentration matched Mouse (G3A1) mAb IgG Isotype Control #5415 (dashed lines). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.