WB, IP, IHC-P, IF-IC
H
Endogenous
200
Rabbit IgG
#O00592
5420
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
For a carrier free (BSA and azide free) version of this product see product #66798.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human PODXL protein.
Background
Podocalyxin is an O-glycosylated and sialylated membrane protein that is closely similar to CD34, the well characterized marker for hematopoietic stem cells (1). In association with several intracellular binding partners, podocalyxin contributes to adhesion and cell morphology (2-4). Podocalyxin is expressed in hematopoietic tissues during stages of active hematopoietic expansion in embryonic development and in more restricted, mainly megakaryocytic lineages in the adult (1,4). Expression also occurs in kidney podocytes, both in embryo and adult, where podocalyxin is essential for organ development and proper kidney function (5-7). A few other cell types that express podocalyxin include some specialized vascular endothelia (8,9) and a subset of neurons (10). It is also aberrantly expressed in a diverse number of cancers, including breast, testicular, pancreatic cancers, and in leukemia (11). Expression is often correlated with poor outcome (11).
- Doyonnas, R. et al. (2005) Blood 105, 4170-8.
- Donowitz, M. et al. (2005) J Physiol 567, 3-11.
- Tan, P.C. et al. (2006) Stem Cells 24, 1150-61.
- Sathyanarayana, P. et al. (2007) Blood 110, 509-18.
- Orlando, R.A. et al. (2001) J Am Soc Nephrol 12, 1589-98.
- Kerjaschki, D. et al. (1984) J Cell Biol 98, 1591-6.
- Doyonnas, R. et al. (2001) J Exp Med 194, 13-27.
- Sassetti, C. et al. (1998) J Exp Med 187, 1965-75.
- Baumhueter, S. et al. (1994) Blood 84, 2554-65.
- Vitureira, N. et al. (2010) PLoS One 5, e12003.
- Nielsen, J.S. and McNagny, K.M. (2009) J Am Soc Nephrol 20, 1669-76.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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