Immunohistochemical analysis of paraffin-embedded normal human pituitary gland using POMC (AH26) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded normal human pituitary gland (left-top) and various paraffin-embedded human tissues: normal colon (middle-top), normal liver (right-top), urothelial carcinoma (left-bottom), non-small cell lung carcinoma (middle-bottom), and ductal breast carcinoma (right-bottom) using POMC (AH26) Mouse mAb. Note the lack of staining in tissues other than the pituitary gland.
Immunohistochemical analysis of paraffin-embedded normal human pituitary gland using POMC (AH26) Mouse mAb (left) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (right).
|Source/Isotype||Mouse IgG1 kappa|
|Immunohistochemistry (Paraffin)||1:800 - 1:3200|
Supplied in 10 mM PBS containing 0.05% BSA and 0.05% sodium azide. Stable for 24 months when stored at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
|DETECTION REAGENT/SUBSTRATE COMPATIBILITY|
|SignalStain® Boost IHC Detection Reagent (HRP, Mouse) #8125||SignalStain® Boost IHC Detection Reagent (AP, Mouse) #31926|
|SignalStain® DAB Substrate Kit #8059||SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713|
|SignalStain® Vivid Purple Peroxidase Substrate Kit #96632|
NOTE: Use of detection reagents other than those specified in this protocol may require further optimization of the primary antibody to account for the different sensitivities of the detection reagents.
posted February 2010
revised April 2020
Protocol Id: 1991
POMC (AH26) Mouse mAb recognizes endogenous levels of total POMC protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids 1-24 of human POMC protein.
Pro-opiomelanocortin (POMC) is a precursor protein expressed in the pituitary and the brain where it is processed into several peptide hormones and neuropeptides. Among these peptides are ACTH, α- and β-MSH, β-and γ-LPH, CLIP, β-endorphin, and N-POMC (1). POMC is involved in hypothalamic circuits regulating feeding behavior and POMC-producing neurons promote satiety (2). POMC neurons are also the target of leptin and insulin for the promotion of the browning of white fat (3).
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