WB, IP, ChIP
H M R Mk
Endogenous
50
Rabbit IgG
#Q03181
5467
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human PPARδ protein.
Background
Peroxisome proliferator-activated receptor-δ (PPARδ, also known as PPARβ or PPARβ/δ) is a widely expressed member of the PPAR nuclear receptor family, which controls lipid homeostasis (1,2). In response to various ligands, PPAR proteins heterodimerize with retinoid X receptors (RXRs) in order to bind DNA and regulate target genes (3,4). PPARδ plays a role in many different biological functions, including cholesterol efflux, embryo implantation, preadipocyte proliferation, and wound healing (5-8). PPARδ has been implicated in colorectal cancer (CRC), as it is normally downregulated by APC, a tumor suppressor frequently knocked out in CRCs (9). More recently, high fat diets have been found to induce PPARδ in intestinal stem cells and progenitors, increasing their tumorigenicity (10). Furthermore, in Huntington's disease (HD) mouse models, it was shown that PPARδ was unable bind to huntingtin protein when mutated, which repressed its function. Agonist-induced activation of PPARδ in HD model mice improved cognitive function and increased survival time (11).
- Kliewer, S.A. et al. (1994) Proc Natl Acad Sci U S A 91, 7355-9.
- Braissant, O. et al. (1996) Endocrinology 137, 354-66.
- Miyata, K.S. et al. (1994) Gene 148, 327-30.
- Forman, B.M. et al. (1997) Proc Natl Acad Sci U S A 94, 4312-7.
- Oliver, W.R. et al. (2001) Proc Natl Acad Sci U S A 98, 5306-11.
- Lim, H. et al. (1999) Genes Dev 13, 1561-74.
- Hansen, J.B. et al. (2001) J Biol Chem 276, 3175-82.
- Tan, N.S. et al. (2001) Genes Dev 15, 3263-77.
- He, T.C. et al. (1999) Cell 99, 335-45.
- Beyaz, S. et al. (2016) Nature 531, 53-8.
- Dickey, A.S. et al. (2016) Nat Med 22, 37-45.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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