Presynaptic Vesicle Cycle Antibody Sampler Kit (#33738) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes	Product #	Quantity	Mol. Wt	Isotype/Source
Complexin-1 (D5Q5H) Rabbit mAb	17700	20 µl	14 kDa	Rabbit IgG
Complexin-1/2 (D8A6E) Rabbit mAb	28070	20 µl	14-16 kDa	Rabbit IgG
Munc18-1 (D4O6V) Rabbit mAb	13414	20 µl	70 kDa	Rabbit IgG
SNAP25 (D7B4) Rabbit mAb	5308	20 µl	25 kDa	Rabbit IgG
Synaptophysin (D8F6H) XP^® Rabbit mAb	36406	20 µl	38 kDa	Rabbit IgG
Synaptotagmin-1 (D33B7) Rabbit mAb	14558	20 µl	60 kDa	Rabbit IgG
Synapsin-1 (D12G5) XP^® Rabbit mAb	5297	20 µl	77 kDa	Rabbit IgG
Syntaxin 1A (D4E2W) Rabbit mAb	18572	20 µl	33 kDa	Rabbit IgG
VAMP2 (D6O1A) Rabbit mAb	13508	20 µl	13 kDa	Rabbit
Anti-rabbit IgG, HRP-linked Antibody	7074	100 µl		Goat

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Presynaptic Vesicle Cycle Antibody Sampler Kit provides an economical means of detecting expression of key synaptic vesicle proteins. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.

Background

The synapse is the unit of information transfer in the brain between neurons (1). Synaptic transfer is initiated by action potential-initiated synaptic vesicles fusion at the presynaptic terminal to release neurotransmitters. The basic molecular machinery that drives all membrane fusion events in all cells include N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins that form a trans-ternary complex between to-be-fused membranes. The zippering up of SNARE proteins provides the energy required for membrane fusion. At the synapse, the SNARE proteins include 25 kDa synaptosome-associated protein (SNAP25), syntaxin 1A (STX1A), and vesicle-associated membrane protein 2 (VAMP2, also called synaptobrevin). Neurotransmitter release, however, is a highly regulated process that occurs in response to action potential-initiated Ca²⁺-influx. Regulation of synaptic vesicle fusion is mediated by SNARE-binding proteins like complexin and the Ca²⁺-associated vesicle protein, synaptotagmin-1, that cooperatively function to initiate Ca²⁺-induced synaptic vesicle fusion. The synaptic vesicle cycle represents a cycle of events that prime the synaptic vesicle before fusion and retrieve fused membrane after full fusion. Synaptic vesicle-associated proteins like synaptophysin and synapsin-1, as well as target membrane proteins like Munc18-1, play a complex role in priming synaptic vesicles to be fusion competent.

Südhof, T.C. and Rothman, J.E. (2009) Science 323, 474-7.

Background References

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.