| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Complexin-1 (D5Q5H) Rabbit mAb | 17700 | 20 µl | 14 kDa | Rabbit IgG |
| Complexin-1/2 (D8A6E) Rabbit mAb | 28070 | 20 µl | 14-16 kDa | Rabbit IgG |
| Munc18-1 (D4O6V) Rabbit mAb | 13414 | 20 µl | 70 kDa | Rabbit IgG |
| SNAP25 (D7B4) Rabbit mAb | 5308 | 20 µl | 25 kDa | Rabbit IgG |
| Synaptophysin (D8F6H) XP® Rabbit mAb | 36406 | 20 µl | 38 kDa | Rabbit IgG |
| Synaptotagmin-1 (D33B7) Rabbit mAb | 14558 | 20 µl | 60 kDa | Rabbit IgG |
| Synapsin-1 (D12G5) XP® Rabbit mAb | 5297 | 20 µl | 77 kDa | Rabbit IgG |
| Syntaxin 1A (D4E2W) Rabbit mAb | 18572 | 20 µl | 33 kDa | Rabbit IgG |
| VAMP2 (D6O1A) Rabbit mAb | 13508 | 20 µl | 13 kDa | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Presynaptic Vesicle Cycle Antibody Sampler Kit provides an economical means of detecting expression of key synaptic vesicle proteins. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
The synapse is the unit of information transfer in the brain between neurons (1). Synaptic transfer is initiated by action potential-initiated synaptic vesicles fusion at the presynaptic terminal to release neurotransmitters. The basic molecular machinery that drives all membrane fusion events in all cells include N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins that form a trans-ternary complex between to-be-fused membranes. The zippering up of SNARE proteins provides the energy required for membrane fusion. At the synapse, the SNARE proteins include 25 kDa synaptosome-associated protein (SNAP25), syntaxin 1A (STX1A), and vesicle-associated membrane protein 2 (VAMP2, also called synaptobrevin). Neurotransmitter release, however, is a highly regulated process that occurs in response to action potential-initiated Ca2+-influx. Regulation of synaptic vesicle fusion is mediated by SNARE-binding proteins like complexin and the Ca2+-associated vesicle protein, synaptotagmin-1, that cooperatively function to initiate Ca2+-induced synaptic vesicle fusion. The synaptic vesicle cycle represents a cycle of events that prime the synaptic vesicle before fusion and retrieve fused membrane after full fusion. Synaptic vesicle-associated proteins like synaptophysin and synapsin-1, as well as target membrane proteins like Munc18-1, play a complex role in priming synaptic vesicles to be fusion competent.
Background References
Trademarks and Patents
Limited Uses
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