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33738
Presynaptic Vesicle Cycle Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Presynaptic Vesicle Cycle Antibody Sampler Kit #33738

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other Image 1 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Confocal immunofluorescent analysis of Neuro-2a (positive, left panels) and NIH/3T3 (negative, right panels) using Complexin-1 (D5Q5H) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red, upper panels). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

other Image 2 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Confocal immunofluorescent analysis of Neuro-2a (left) and NIH/3T3 (right) cells, using Complexin-1/2 (D8A6E) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

other Image 3 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Western blot analysis of extracts from mouse brain, rat brain, and human cortex using Munc18-1 (D4O6V) Rabbit mAb.

other Image 4 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Western blot analysis of extracts from mouse and rat brain using SNAP25 (D7B4) Rabbit mAb.

other Image 5 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Confocal immunofluorescent analysis of mouse retina using Synaptophysin (D8F6H) XP® Rabbit mAb (green), Neurofilament-L (DA2) Mouse mAb #2835 (yellow) and Lamin A/C (4C11) Mouse mAb #4777 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

other Image 6 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Confocal immunofluorescent analysis of mouse retina using Synaptotagmin-1 (D33B7) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

other Image 7 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Confocal immunofluorescent analysis of mouse brain using Synapsin-1 (D12G5) XP® Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

other Image 8 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Western blot analysis of extracts from mouse brain, mouse lung, and rat brain using Syntaxin 1A (D4E2W) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

other Image 9 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Confocal immunofluorescent analysis of normal rat retina using VAMP2 (D6O1A) Rabbit mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

other Image 10 - Presynaptic Vesicle Cycle Antibody Sampler Kit

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

other Image 11 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunoprecipitation of Complexin-1 from Neuro-2a cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 and lane 3 is Complexin-1 (D5Q5H) Rabbit mAb. Western blot was performed using Complexin-1 (D5Q5H) Rabbit mAb.

other Image 12 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunoprecipitation of complexin-1/2 from Neuro-2A cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Complexin-1/2 (D8A6E) Rabbit mAb. Western blot analysis was performed using Complexin-1/2 (D8A6E) Rabbit mAb.

other Image 13 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Confocal immunofluorescent analysis of mouse brain using Synaptophysin (D8F6H) XP® Rabbit mAb (green), Neurofilament-L (DA2) Mouse mAb #2835 (yellow) and Lamin A/C (4C11) Mouse #4777 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

other Image 14 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Western blot analysis of extracts from mouse and rat brain using Synaptotagmin-1 (D33B7) Rabbit mAb.

other Image 15 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunohistochemical analysis of paraffin-embedded human cerebellum using Synapsin-1 (D12G5) XP® Rabbit mAb.

other Image 16 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Confocal immunofluorescent analysis of PC-12 cells using VAMP2 (D6O1A) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

other Image 17 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Western blot analysis of extracts from various cells lines and tissues using Complexin-1 (D5Q5H) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

other Image 18 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Western blot analysis of extracts from Neuro-2a, NIH/3T3, and mouse brain, using Complexin-1/2 (D8A6E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

other Image 19 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunohistochemical analysis of paraffin-embedded Neuro-2a cell pellet (left, positive) or C2C12 cell pellet (right, negative) using Synaptophysin (D8F6H) XP® Rabbit mAb.

other Image 20 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunohistochemical analysis of paraffin-embedded mouse cortex using Synapsin-1 (D12G5) XP® Rabbit mAb.

other Image 21 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunohistochemical analysis of paraffin-embedded human colon using VAMP2 (D6O1A) Rabbit mAb.

other Image 22 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full length human complexin-1 (hCPLX-1-Myc; +) or complexin-2 (hCPLX-2-Myc; +), using Complexin-1 (D5Q5H) Rabbit mAb (upper), and Myc-Tag (71D10) Rabbit mAb #2278 (lower).

other Image 23 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human complexin-1 (hCPLX-1-Myc; +) or complexin-2 (hCPLX-2-Myc; +), using Complexin-1/2 (D8A6E) Rabbit mAb (upper), and Myc-Tag (71D10) Rabbit mAb #2278 (lower).

other Image 24 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunohistochemical analysis of paraffin-embedded mouse cerebellum using Synaptophysin (D8F6H) XP® Rabbit mAb.

other Image 25 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunohistochemical analysis of paraffin-embedded rat retina using Synapsin-1 (D12G5) XP® Rabbit mAb.

other Image 26 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunohistochemical analysis of paraffin-embedded rat kidney using VAMP2 (D6O1A) Rabbit mAb.

other Image 27 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunohistochemical analysis of paraffin-embedded mouse colon using Synaptophysin (D8F6H) XP® Rabbit mAb.

other Image 28 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Western blot analysis of extracts from CAD cells and neonatal mouse brain using Synapsin-1 (D12G5) XP® Rabbit mAb.

other Image 29 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunohistochemical analysis of paraffin-embedded mouse pancreas using VAMP2 (D6O1A) Rabbit mAb.

other Image 30 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Synaptophysin (D8F6H) XP® Rabbit mAb.

other Image 31 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunoprecipitation of VAMP2 from Neuro-2a cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or VAMP2 (D6O1A) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using VAMP2 (D6O1A) Rabbit mAb.

other Image 32 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Synaptophysin (D8F6H) XP® Rabbit mAb.

other Image 33 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Western blot analysis of extracts from various cell lines and tissues using VAMP2 (D6O1A) Rabbit mAb.

other Image 34 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Immunohistochemical analysis of paraffin-embedded mouse pancreas using Synaptophysin (D8F6H) XP® Rabbit mAb.

other Image 35 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human VAMP1 (hVAMP1-Myc; +) or VAMP2 (hVAMP2-Myc; +), using VAMP2 (D6O1A) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).

other Image 36 - Presynaptic Vesicle Cycle Antibody Sampler Kit

Western blot analysis of extracts from mouse and rat tissue using Synaptophysin (D8F6H) XP® Rabbit mAb (upper) and B-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 33738T
Product # Size Price
33738T
1 Kit  (9 x 20 µl) $ 609

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Complexin-1 (D5Q5H) Rabbit mAb 17700 20 µl
  • WB
  • IP
  • IF
H M R 14 Rabbit IgG
Complexin-1/2 (D8A6E) Rabbit mAb 28070 20 µl
  • WB
  • IP
  • IF
H M R 14-16 Rabbit IgG
Munc18-1 (D4O6V) Rabbit mAb 13414 20 µl
  • WB
H M R 70 Rabbit IgG
SNAP25 (D7B4) Rabbit mAb 5308 20 µl
  • WB
  • IP
H M R 25 Rabbit IgG
Synaptophysin (D8F6H) XP® Rabbit mAb 36406 20 µl
  • WB
  • IHC
  • IF
H M R 38 Rabbit IgG
Synaptotagmin-1 (D33B7) Rabbit mAb 14558 20 µl
  • WB
  • IP
  • IF
H M R 60 Rabbit IgG
Synapsin-1 (D12G5) XP® Rabbit mAb 5297 20 µl
  • WB
  • IP
  • IHC
  • IF
H M R 77 Rabbit IgG
Syntaxin 1A (D4E2W) Rabbit mAb 18572 20 µl
  • WB
H M R 33 Rabbit IgG
VAMP2 (D6O1A) Rabbit mAb 13508 20 µl
  • WB
  • IP
  • IHC
  • IF
H M R 13 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Presynaptic Vesicle Cycle Antibody Sampler Kit provides an economical means of detecting expression of key synaptic vesicle proteins. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Presynaptic Vesicle Cycle Antibody Sampler Kit detects endogenous levels of its target protein. VAMP2 (D6O1A) Rabbit mAb does not cross-react with VAMP1 protein and recognizes VAMP2 protein by IF-IC analysis in mouse and rat cells. Complexin-1/2 (D8A6E) Rabbit mAb recognizes endogenous levels of total complexin-1 and complexin-2 protein. Complexin-1 (D5Q5H) Rabbit mAb does not cross-react with complexin-2. Synaptotagmin-1 (D33B7) Rabbit mAb may also cross-react with an unidentified protein of approximately 45 kDa. The antigen for Synapsin-1 (D12G5) XP® Rabbit mAb is 100% conserved between human synapsin-1a and synapsin-1b.

Source / Purification

Complexin-1 (D5Q5H) Rabbit mAb is produced by immunizing recombinant protein specific to human complexin-1 protein. Complexin-1/2 (D8A6E) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Pro125 of human complexin-2 protein. VAMP2 (D6O1A) Rabbit mAb is produced by immunizing recombinant protein specific to the amino terminus of human VAMP2 protein. All other monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to Asp110 of human SNAP25, Val237 of human syntaxin 1A, Gly299 of human synaptophysin, Arg400 of human synaptotagmin-1, Gln483 of human synapsin-1, and Tyr157 of human Munc18-1.

Background

The synapse is the unit of information transfer in the brain between neurons (1). Synaptic transfer is initiated by action potential-initiated synaptic vesicles fusion at the presynaptic terminal to release neurotransmitters. The basic molecular machinery that drives all membrane fusion events in all cells include N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins that form a trans-ternary complex between to-be-fused membranes. The zippering up of SNARE proteins provides the energy required for membrane fusion. At the synapse, the SNARE proteins include 25 kDa synaptosome-associated protein (SNAP25), syntaxin 1A (STX1A), and vesicle-associated membrane protein 2 (VAMP2, also called synaptobrevin). Neurotransmitter release, however, is a highly regulated process that occurs in response to action potential-initiated Ca2+-influx. Regulation of synaptic vesicle fusion is mediated by SNARE-binding proteins like complexin and the Ca2+-associated vesicle protein, synaptotagmin-1, that cooperatively function to initiate Ca2+-induced synaptic vesicle fusion. The synaptic vesicle cycle represents a cycle of events that prime the synaptic vesicle before fusion and retrieve fused membrane after full fusion. Synaptic vesicle-associated proteins like synaptophysin and synapsin-1, as well as target membrane proteins like Munc18-1, play a complex role in priming synaptic vesicles to be fusion competent.   

  1. Südhof, T.C. and Rothman, J.E. (2009) Science 323, 474-7.

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