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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Bad (D24A9) Rabbit mAb 9239 20 µl
Western Blotting
H M R Mk 23 Rabbit IgG
Phospho-Bad (Ser112) (40A9) Rabbit mAb 5284 20 µl
Western Blotting Immunohistochemistry Flow Cytometry
H M R Mk 23 Rabbit IgG
Bax (D2E11) Rabbit mAb 5023 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H 20 Rabbit IgG
Bik Antibody 4592 20 µl
Western Blotting
H 20 Rabbit 
Bim (C34C5) Rabbit mAb 2933 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R 12, 15, 23 Rabbit IgG
BID Antibody (Human Specific) 2002 20 µl
Western Blotting Immunoprecipitation
H 15, 22 Rabbit 
Bak (D4E4) Rabbit mAb 12105 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk 25 Rabbit IgG
Puma (D30C10) Rabbit mAb 12450 20 µl
Western Blotting Immunoprecipitation
H 23 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting

Product Description

The Pro-Apoptosis Bcl-2 Family Antibody Sampler Kit provides an economical means to examine several members of the Bcl-2 family and their activation status. The kit contains enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.

Specificity / Sensitivity

Each antibody in the Pro- Apoptosis Bcl-2 Family Antibody Sampler Kit recognizes only its specific target. The antibodies do not cross-react with other Bcl-2 family members. Phospho-Bad (Ser112) (40A9) Rabbit mAb detects endogenous levels of Bad only when phosphorylated at Ser112 (mouse), Ser75 (human), or Ser113 (rat). Puma (D30C10) Rabbit mAb recognizes endogenous levels of total Puma protein but also cross-reacts with a protein of unknown origin at 60 kDa.

Source / Purification

Phospho-Bad (Ser112) (40A9) Rabbit mAb is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser112 of mouse Bad. Total Bad, Bax, Bim, Bak and Puma monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro102 of human Bad, Leu45 of human Bax, Pro25 of human Bim, Gly75 of human Bak, or near the carboxy terminus of human Puma. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the amino-terminus of human Bik or residues surrounding the cleavage site of human BID. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2 homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c (1-3). Four BH domains have been identified (BH1-4) that mediate protein interactions. The family can be separated into three groups based upon function and sequence homology: pro-survival members include Bcl-2, Bcl-xL, Mcl-1, A1 and Bcl-w; pro-apoptotic proteins include Bax, Bak and Bok; and "BH3 only" proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa and Hrk. Interactions between death-promoting and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate (4). Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the "BH3-only members" can bind to and antagonize the pro-survival proteins leading to increased apoptosis (5). While some redundancy of this system likely exists, tissue specificity, transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles.

Bad is a pro-apoptotic member of the Bcl-2 family that can displace Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death (6,7). Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (7). Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-2 and Bcl-xL (7). Akt has been shown to promote cell survival via its ability to phosphorylate Bad at Ser136 (8,9). Ser112 has been shown to be the substrate in vivo and in vitro of p90RSK (10,11) and mitochondria-anchored PKA (12).

1.  Cory, S. et al. (2003) Oncogene 22, 8590-607.

2.  Antonsson, B. and Martinou, J.C. (2000) Exp Cell Res 256, 50-7.

3.  Sharpe, J.C. et al. (2004) Biochim Biophys Acta 1644, 107-13.

4.  Korsmeyer, S.J. et al. (1993) Semin Cancer Biol 4, 327-32.

5.  Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74.

6.  Yang, E. et al. (1995) Cell 80, 285-291.

7.  Zha, J. et al. (1996) Cell 87, 619-628.

8.  Datta, S. R. et al. (1997) Cell 91, 231-241.

9.  Peso, L. et al. (1997) Science 278, 687-689.

10.  Bonni, A. et al. (1999) Science 286, 1358-1362.

11.  Tan, Y. et al. (1999) J. Bio. Chem. 274, 34859-34867.

12.  Harada, H. et al. (1999) Mol. Cell 3, 413-422.

Entrez-Gene Id 572 , 578 , 581 , 637 , 638 , 10018 , 27113
Swiss-Prot Acc. Q92934 , Q16611 , Q07812 , P55957 , Q13323 , O43521 , Q9BXH1

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

Pro-Apoptosis Bcl-2 Family Antibody Sampler Kit