WB, IP, ChIP, eCLIP
H M R Mk
Endogenous
57, 59
Rabbit IgG
#P26599-1
5725
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:50 |
| eCLIP | 1:200 |
For more information about the RBP-eCLIP service please visit Eclipsebio.
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val10 of human PTBP protein.
Background
PTBP1 and PTBP2 are highly related pre-mRNA binding proteins that silence the splicing of alternative exons (1,2). While PTBP1 is widely expressed, PTBP2, also known as neural PTB or nPTB, is expressed in post-mitotic neurons. In other cell types, PTBP2 transcripts are alternatively spliced by PTBP1, which leads to nonsense-mediated decay (3). PTBP1 and 2 bind to similar regions across the transcriptome, but PTBP1 has a stronger repressive property likely due to cofactors such as Raver1 and Matrin3 (4-6). Overexpression of PTBPs in various cancers has been observed, leading to alternative splicing of key proteins in oncogenic pathways (6-9).
- Amir-Ahmady, B. et al. (2005) RNA 11, 699-716.
- Oberstrass, F.C. et al. (2005) Science 309, 2054-7.
- Boutz, P.L. et al. (2007) Genes Dev 21, 1636-52.
- Keppetipola, N.M. et al. (2016) RNA 22, 1172-80.
- Vuong, J.K. et al. (2016) Cell Rep 17, 2766-2775.
- Minami, K. et al. (2017) Oncotarget 8, 33064-33077.
- Wang, Z.N. et al. (2017) Oncotarget 8, 36185-36202.
- Uemura, Y. et al. (2017) Genes Cells 22, 785-798.
- Bielli, P. et al. (2018) Clin Cancer Res 24, 5422-5432.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP eCLIP: eCLIP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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