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PhosphoSitePlus® Resource

  • Additional protein information
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100, 110, 210 Mouse 

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

PTPmu Mouse mAb detects endogenous levels of total PTPmu protein. This antibody does not cross react with other receptor tyrosine phosphatases.

Species predicted to react based on 100% sequence homology: Mouse

Source / Purification

Monoclonal antibody (isotype: IgG2a) is produced by immunizing mice with a synthetic peptide corresponding to the amino-terminal residues of human PTPmu.

Receptor tyrosine phosphatase PTPmu has an extracellular segment characteristic of adhesion molecules: an MEM domain, an Ig domain and four fibronectin III like (FN III) repeats (1,2). PTPmu is proteolytically cleaved into two noncovalently associated fragments: one is the extracellular domain, the other includes the transmembrane and the intracellular catalytic domains. Both fragments are approximately 100 kDa (3). The extracellular domain mediates cell-cell adhesion in a homophilic, Ca2+ independent manner (1,2). PTPmu associates with multiple cadherins (4). It is able to restore E-cadherin-dependent adhesion in human prostate cancer, and is required for N-cadherin-mediated neurite outgrowth (5,6). The phosphatase activity seems to be essential for the latter function but is dispensable for the former (5,6). PTPmu also associates with and recruits a scaffold protein, RACK (receptor for activated protein C kinase), to cell-cell contact sites (7). Both PKCdelta and src seem to be involved in this process (6,7).

1.  Gebbink, M. F. et al. (1993) J. Biol. Chem. 268, 16101-16104.

2.  Brady-Kalnay, S.M. and Tonks, N.K. (1994) J. Biol. Chem. 269, 28472-28477.

3.  Brady-Kalnay, S. M. et al. (1998) J. Cell Biol. 141, 287-296.

4.  Hellberg, C. B. et al. (2002) J. Biol. Chem. 277, 11165-11173.

5.  Burden-Gulley, S.M. and Brady-Kalnay, S.M. (1999) J. Cell Biol. 144, 1323-1336.

6.  Mourton, T. et al. (2001) J. Biol. Chem. 276, 14896-14901.

Entrez-Gene Id 5797
Swiss-Prot Acc. P28827

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PTPmu (BK2) Mouse mAb