Revision 3

#50340

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H M R Mk

Sensitivity:
Endogenous

MW (kDa):
97

Source/Isotype:
Rabbit IgG

UniProt ID:
#P11216

Entrez-Gene Id:
5834

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

PYGB (E3W5W) Rabbit Monoclonal Antibody recognizes endogenous levels of total PYGB protein. This antibody does not cross-react with PYGL or PYGM.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro836 of human PYGB protein.

Background

Glycogen phosphorylases constitute a family of metabolic enzymes that catalyze the rate-limiting step in the release of glucose-1-phosphate (G1P) from tissue glycogen stores (1,2). There are three distinct glycogen phosphorylases in vertebrates, encoded by distinct genes and characterized primarily by the tissues in which they are predominantly expressed: PYGL (liver form), PYGB (brain form), and PYGM (muscle form). All three forms are expressed as inactive monomers, which require homodimerization and site-specific phosphorylation for activation, with additional regulation possible via other post-translational modifications such as O-GlycNAcylation (3). Due to their critical role in regulating glycogen catabolism, mutations or other perturbations that impact glycogen phosphorylase activity have been identified as contributing factors to metabolic diseases (e.g., glycogen storage diseases) and other diseases with metabolic dependencies, including cancer (4,5). While PYGL is expressed predominantly in the liver, it is understood to regulate systemic (blood) glucose levels via the catabolism of liver glycogen stores, whereas PYGB and PYGM regulate glycogen metabolism specifically within brain and skeletal muscle tissues, respectively (6,7).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human M: Mouse R: Rat Mk: Monkey

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.