WB, IP, IF-IC
H M Mk
Endogenous
85
Rabbit IgG
#Q6WKZ4
80223
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu248 of human Rab11FIP1 protein.
Background
Rab11 family proteins consist of closely related Rab11a, Rab11b, and Rab25. They are small GTPases thought to play an essential role in regulating endocytic membrane traffic (1,2). The GTP-bound active state Rab11 proteins interact with the Rab11 family interacting proteins (Rab11FIPs) via a conserved carboxy-terminal Rab11-binding domain (3,4). At least six members of the Rab11FIPs have been identified. Based on sequence homology and domain structures, Rab11FIP1, Rab11FIP2 and Rab11FIP5 are categorized as class I subfamily members, whereas Rab11FIP3 and Rab11FIP4 belong to the class II subfamily that bind Arf6 and Rab11 proteins (5-7). Research studies have implicated Rab11 family proteins and their interacting effectors in carcinogenesis (8,9).
- Bock, J.B. et al. (2001) Nature 409, 839-41.
- Ullrich, O. et al. (1996) J Cell Biol 135, 913-24.
- Hales, C.M. et al. (2001) J Biol Chem 276, 39067-75.
- Prekeris, R. et al. (2001) J Biol Chem 276, 38966-70.
- Meyers, J.M. and Prekeris, R. (2002) J Biol Chem 277, 49003-10.
- Lindsay, A.J. and McCaffrey, M.W. (2004) J Cell Sci 117, 4365-75.
- Hickson, G.R. et al. (2003) Mol Biol Cell 14, 2908-20.
- Zhang, J. et al. (2009) J Clin Invest 119, 2171-83.
- Muller, P.A. et al. (2009) Cell 139, 1327-41.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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