WB
H M R
Endogenous
24
Rabbit
#Q15286
11021
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp124 of human Rab35 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Rab35 belongs to the small GTPase superfamily. By interacting with its specific GEF or GAP, Rab35 regulates cargo-specific endocytosis at the vesicle recycling step or mediates exocytosis at the exosome docking/tethering step (1-4). During cytokinesis, Rab35 interacts with OCRL phosphatase and is essential for maintaining intercellular bridge stability and abscission by controlling the concentration of phosphatidylinositol 4,5-bisphosphate (PIP2) and SEPT2 localization at the intercellular bridge (5,6). Rab35 also plays a role in actin assembly and the recruitment of Cdc42 and Rac1 to the site of filopodium by its direct interaction with actin-bundling protein fascin and actin-binding protein connecdenn 3 (7-9).
- Patino-Lopez, G. et al. (2008) J Biol Chem 283, 18323-30.
- Sato, M. et al. (2008) EMBO J 27, 1183-96.
- Marat, A.L. and McPherson, P.S. (2010) J Biol Chem 285, 10627-37.
- Hsu, C. et al. (2010) J Cell Biol 189, 223-32.
- Kouranti, I. et al. (2006) Curr Biol 16, 1719-25.
- Dambournet, D. et al. (2011) Nat Cell Biol 13, 981-8.
- Zhang, J. et al. (2009) Science 325, 1250-4.
- Marat, A.L. et al. (2012) Mol Biol Cell 23, 163-75.
- Shim, J. et al. (2010) Mol Cell Biol 30, 1421-33.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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