WB, IP
H M R
Endogenous
60-80
Rabbit IgG
#Q9UJ41
27342
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rabex-5 protein.
Background
Rabex-5, also called RabGEF1 and RAP1, was identified as a guanine nucleotide exchange factor (GEF) for Rab5, a member of the Ras superfamily of small Rab GTPases (1). Rabex-5 generates the GTP-bound active form of Rab5 and forms a tight association with its effector protein Rabaptin-5 (2). This complex localizes to endosomal membranes where it functions as a key regulator of vesicular trafficking during early endocytosis (3,4). Rabex-5 is also monoubiquitinated and has ubiquitin ligase activity that regulates its recruitment to early endosomes (5,6). The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis (5). Through its control of endosomal trafficking and endocytosis, Rabex-5 has been shown to negatively regulate NGF-mediated neurite outgrowth (7) as well as FcεRI-dependent mast cell activation (8).
- Horiuchi, H. et al. (1997) Cell 90, 1149-59.
- Lippé, R. et al. (2001) Mol Biol Cell 12, 2219-28.
- Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
- van der Bliek, A.M. (2005) Nat Cell Biol 7, 548-50.
- Mattera, R. et al. (2006) J Biol Chem 281, 6874-83.
- Mattera, R. and Bonifacino, J.S. (2008) EMBO J 27, 2484-94.
- Liu, J. et al. (2007) Mol Biol Cell 18, 1375-84.
- Tam, S.Y. et al. (2004) Nat Immunol 5, 844-52.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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