WB
H
Endogenous
27
Rabbit
#P43487
5902
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr193 of human RanBP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
RanBP1 is a Ran binding protein that functions in nuclear trafficking for both nuclear import and export (1-3). Its protein sequence contains a Ran binding domain and a C-terminal nuclear export signal, which maintains its cytoplasmic localization (2,3). During nuclear export, RanBP1 forms a complex with RanGTP and CRM1/cargo, leading to dissociation of cargo from CRM1 (2,4). RanBP1 further stimulates RanGTP-RanGAP1 association to facilitate RanGTP hydrolysis and the generation of RanGDP to complete the final steps of nuclear export (5). During nuclear import, RanBP1 stabilizes the formation of a RanGDP-importin/NLS receptor-RanBP1 complex. This complex regulates the release of imported cargo into the nucleus (6,7). In addition to nuclear trafficking, RanBP1 also controls RanGTP distribution along mitotic microtubules, which localizes critical factors, such as cyclin B1 and HURP, to mitotic microtubles and regulates chromosome segregation (8,9). In vivo knock down or overexpression of RanBP1 has been shown to affect cellular ciliogenesis by regulating the local RanGTP concentation at the base of cilia (10).
- Bischoff, F.R. et al. (1995) EMBO J 14, 705-15.
- Kehlenbach, R.H. et al. (1999) J Cell Biol 145, 645-57.
- Richards, S.A. et al. (1996) J Cell Biol 134, 1157-68.
- Koyama, M. and Matsuura, Y. (2010) EMBO J 29, 2002-13.
- Seewald, M.J. et al. (2003) Mol Cell Biol 23, 8124-36.
- Chi, N.C. et al. (1996) J Cell Biol 135, 559-69.
- Lonhienne, T.G. et al. (2009) J Biol Chem 284, 22549-58.
- Tedeschi, A. et al. (2007) J Cell Sci 120, 3748-61.
- Di Fiore, B. et al. (2003) J Cell Sci 116, 3399-411.
- Fan, S. et al. (2011) Mol Biol Cell 22, 4539-48.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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