WB, IP
H
Transfected Only
35-45
Rabbit
#O14788
8600
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Monkey, Bovine, Pig
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RANK Ligand, within the cytoplasmic region. Antibody was purified by protein A and peptide affinity chromatography.
Background
RANK (receptor activator of NF-κB) is a member of the tumor necrosis factor (TNF) receptor subfamily that is activated by its ligand, RANKL (TRANCE/OPGL/ODF), to promote survival of dendritic cells and differentiation of osteoclasts (1-4). Although RANK is widely expressed, its cell surface expression may be more restricted to dendritic cells and foreskin fibroblasts (1). RANK contains a 383-amino acid intracellular domain that associates with specific members of the TRAF family to NF-κB and JNK activation (1,5). RANKL/RANK signaling may also lead to survival signaling through activation of the Akt pathway and an upregulation of survival proteins, including Bcl-xL (2,6). RANK signaling has been implicated as a potential therapeutic to inhibit bone loss and arthritis (7,8).
RANKL (1), also named TNF-related activation-induced cytokine (TRANCE) (2,9), osteoprotegerin ligand (OPGL) (3), osteoclast differentiation factor (ODF) (4), and TNFSF11, is a type II transmembrane protein of the TNF family that exists as both a membrane-bound and soluble form. It is an essential regulator of immune function and bone development and homeostasis (7,10,11). RANKL is predominately expressed in activated T cells, as well as the thymus, lymph node, and bone marrow and promotes dendritic cell survival. Deletion of RANKL in mice leads to severe osteoporosis with a loss of osteoclasts, defects in T and B cell differentiation, loss of lymph node development, and mammary gland development during pregnancy (12-14).
- Anderson, D.M. et al. (1997) Nature 390, 175-9.
- Wong, B.R. et al. (1997) J. Exp. Med. 186, 2075-80.
- Lacey, D.L. et al. (1998) Cell 93, 165-76.
- Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 3597-602.
- Darnay, B.G. et al. (1998) J. Biol. Chem. 273, 20551-5.
- Wong, B.R. et al. (1999) Mol. Cell 4, 1041-9.
- Walsh, M.C. and Choi, Y. Cytokine Growth Factor Rev. 14, 251-63.
- Nakashima, T. et al. (2003) Curr. Opin. Rheumatol. 15, 280-7.
- Wong, B.R. et al. (1997) J Biol Chem 272, 25190-4.
- Hofbauer, L.C. (1999) Eur J Endocrinol 141, 195-210.
- Theill, L.E. et al. (2002) Annu Rev Immunol 20, 795-823.
- Mizuno, A. et al. (1998) Biochem Biophys Res Commun 247, 610-5.
- Kong, Y.Y. et al. (1999) Nature 397, 315-23.
- Fata, J.E. et al. (2000) Cell 103, 41-50.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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