WB, IP
H
Endogenous
150
Rabbit IgG
#Q9UJF2
9462
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro69 of human RASAL2 protein.
Background
Small GTPases act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. RASAL2 was initially identified as a GAP for the small GTPase, Ras, and later shown to interact with the Rho-GEF ECT2, and to regulate Rho activity in human astrocytoma cells (1).
Researchers have implicated RASAL2 as a suppressor of migration and metastasis in human cancer (2), and have shown that RASAL2 downregulation promotes epithelial-mesenchymal transition and metastasis in ovarian cancer (3) and lung cancer (4). Conversely, other research studies show that RASAL2 can be oncogenic in triple negative breast cancer through activation of Rac1 signaling (5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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