WB
H M R Mk
Endogenous
110
Rabbit IgG
#O95980
8434
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile913 of human RECK protein.
Background
RECK (reversion-inducing cysteine-rich protein with Kazal motif) is a GPI-anchored membrane glycoprotein that negatively regulates members of the matrix metalloproteinase (MMP) family and functions as a suppressor of transformation (1,2). Its function in MMP inhibition makes RECK a crucial factor in the regulation of extracellular matrix formation and stability during development (2-4). RECK has also been linked to the regulation of other extracellar matrix proteases such as ADAM10 and CD13 and functions in modulating target protein endocytosis and Notch signaling (5,6). RECK is widely expressed in normal tissue and decreased expression of RECK due to promoter methylation has been correlated with tumor transformation, angiogenesis and metastasis (1,7-9). Therefore, loss of RECK expression serves as a prognostic hallmark for cancer malignancy (10,11)
- Takahashi, C. et al. (1998) Proc Natl Acad Sci USA 95, 13221-6.
- Oh, J. et al. (2001) Cell 107, 789-800.
- Kondo, S. et al. (2007) J Cell Sci 120, 849-57.
- Echizenya, M. et al. (2005) Oncogene 24, 5850-7.
- Miki, T. et al. (2007) J Biol Chem 282, 12341-52.
- Muraguchi, T. et al. (2007) Nat Neurosci 10, 838-45.
- Noda, M. et al. Cancer Metastasis Rev 22, 167-75.
- Long, N.K. et al. (2008) Oral Oncol 44, 1052-8.
- Chang, H.C. et al. (2007) Cancer Sci 98, 169-73.
- Clark, J.C. et al. (2007) Cancer Metastasis Rev 26, 675-83.
- Noda, M. and Takahashi, C. (2007) Cancer Sci 98, 1659-65.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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