WB
H
Endogenous
97
Rabbit IgG
#Q6PJF5
79651
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys36 of human RHBDF2 protein.
Background
Inactive rhomboid factor 2 (RHBDF2), also known as RHBDL6 or iRhom2, is a proteolytically inactive member of the transmembrane family of rhomboid serine protease family (1). Inactive rhomboid family members have been implicated in several physiological and pathophysiological conditions, including wound healing, cancer, neurodegenerative diseases, and inflammatory disorders (reviewed in 2). RHBDF2 facilitates endoplasmic reticulum trafficking of the metalloprotease ADAM17 (also known as TACE) to the membrane, thereby promoting EGF family growth factor and cytokine shedding and signaling (1). Loss of RHBDF2 results in significantly reduced secretion of ligand-mediated secretion of these factors. Inherited dominant mutations in the RHBDF2 gene are the genetic basis for the cancer-susceptibility syndrome tylosis with esophageal cancer (TOC) (3). Elevated expression of RHBDF2 in oral squamous carcinoma is associated with tumor progression and poor patient prognosis (4). The E3 ubiquitin ligase TRIM31 directly targets RHBDF2 and facilitates its proteasomal degradation (5). Hepatocyte specific ablation of TRIM31 facilitates phenotypes of non-alcoholic fatty liver disease (5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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