WB, IP, IHC-P, FC-FP
H M R
Endogenous
43-48
Rabbit IgG
#Q13761
864
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RUNX3 protein.
Background
Runt-related transcription factor 3 (RUNX3, AML2), a member of the Runt family of transcription factors, plays an important role in the suppression of gastric epithelium cell proliferation (1), dorsal root ganglia neurogenesis (2), and T cell differentiation (3,4). RUNX3 is also involved in caspase-3-dependent apoptosis (5). Protein complexes containing RUNX3 and various transcription factors, such as Smads or β-catenin/TCF4, have tumor suppressor activity and regulate downstream target gene transcription (6,7). While typically localized to the nucleus, RUNX3 can be tyrosine phosphorylated and located in the cytoplasm of many cancer cells. This mislocalization of RUNX3 abolishes its tumor suppressor function and contributes to tumorigenesis (8). Research studies indicate that gene silencing or protein mislocalization inactivates RUNX3 in more than 80% of gastric cancers and other cancer types (1,9,10).
- Li, Q.L. et al. (2002) Cell 109, 113-24.
- Inoue, K. et al. (2002) Nat Neurosci 5, 946-54.
- Taniuchi, I. et al. (2002) Cell 111, 621-33.
- Woolf, E. et al. (2007) Dev Biol 303, 703-14.
- Zhai, F.X. et al. (2012) J Cancer Res Clin Oncol 138, 439-49.
- Chi, X.Z. et al. (2005) Mol Cell Biol 25, 8097-107.
- Ito, K. et al. (2008) Cancer Cell 14, 226-37.
- Goh, Y.M. et al. (2010) J Biol Chem 285, 10122-9.
- Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-87.
- Ito, K. et al. (2005) Cancer Res 65, 7743-50.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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