WB
Vir
Transfected Only
20-30
Rabbit
#P59596
1489672
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Virus
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly211 of SARS-CoV-1 membrane protein. Antibodies are purified by peptide affinity chromatography.
Background
The cause of the SARS epidemic in 2003 was a novel pathogenic coronavirus, originally termed SARS-CoV (severe acute respiratory syndrome coronavirus) (1). Following the 2019 emergence of SARS-CoV-2 and the COVID-19 pandemic, SARS-CoV is now often referred to as SARS-CoV-1. SARS-CoV-1 is a member of the Coronaviridae family of single-stranded RNA viruses (2). The genome of SARS-CoV-1 is relatively large among viruses (~30 kDa); it encodes four key structural proteins (spike, envelope, membrane, and nucleocapsid) that make up the virion particle, in addition to a variety of smaller open reading frames (ORFs) that encode the accessory proteins required for viral replication (3). The SARS-CoV-1 membrane protein (M) is the most abundant protein in the viral envelope; in addition to its structural role, it plays an important role in virus-host interactions and the viral lifecycle (4). Notably, studies have shown that membrane proteins from both SARS-CoV-1 and SARS-CoV-2 may induce apoptosis in host cells, suggesting one mechanism by which SARS coronaviruses cause tissue damage upon infection (5,6).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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