Immunohistochemical analysis of paraffin-embedded human breast carcinoma using DF1 (D8G6H) Rabbit mAb (IHC-Specific).Learn more about how we get our images.
mmunohistochemical analysis of paraffin-embedded human ovarian carcinoma using SDF1 (D8G6H) Rabbit mAb (IHC-Specific).Learn more about how we get our images.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using DF1 (D8G6H) Rabbit mAb (IHC-Specific).Learn more about how we get our images.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
posted February 2010
revised March 2016
Protocol Id: 283
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
SDF1 (D8G6H) Rabbit mAb (IHC-Specific) recognizes endogenous levels of total SDF1 protein by immunohistochemistry on paraffin-embedded tissue sections. This antibody recognizes both SDF1α and SDF1β.
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human SDF1 protein.
The stromal cell derived factor 1 (SDF1, CXCL12) is a small, pro-inflammatory chemoattractant cytokine that regulates leukocyte trafficking through interactions with its cognate 7-transmembrane G protein-coupled receptors (1). The SDF1 receptor, CXCR4, also serves as a coreceptor for the entry of human immunodeficiency virus into target cells (2). SDF1 may regulate homing and maintenance of CXCR4-expressing stem or progenitor cells, including embryonic and many somatic stem cells (3,4). Many cancer cells express CXCR4, suggesting that SDF1 plays a role in cancer metastasis (5,6). Alternative splicing and differential processing during maturation produce a pair of SDF1 isoforms (SDF1α and SDF1β) that have different properties and biological activities (7). Additional isoforms of SDF1 have been reported (8,9).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. SignalStain is a trademark of Cell Signaling Technology, Inc. Tween is a registered trademark of ICI Americas, Inc.
Explore pathways related to this product.
|97958S||100 µl (200 sections)||$ 255.0|