Revision 1
#25501
Store at -20C
SET1/COMPASS Antibody Sampler Kit
1 Kit
(8 x 20 microliters)
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| SET1A (D3V9S) Rabbit Monoclonal Antibody | 61702 | 20 µl | 300 kDa | Rabbit IgG |
| SET1B (D1U5D) Rabbit Monoclonal Antibody | 44922 | 20 µl | 320 kDa | Rabbit IgG |
| MLL1 (D2M7U) Rabbit Monoclonal Antibody (Amino-terminal Antigen) | 14689 | 20 µl | 300 kDa | Rabbit IgG |
| MLL1 (D6G8N) Rabbit Monoclonal Antibody (Carboxy-terminal Antigen) | 14197 | 20 µl | 180 kDa | Rabbit IgG |
| MLL2/KMT2B (D6X2E) Rabbit Monoclonal Antibody (Carboxy-terminal Antigen) | 63735 | 20 µl | 80 kDa | Rabbit IgG |
| WDR5 (D9E1I) Rabbit Monoclonal Antibody | 13105 | 20 µl | 37 kDa | Rabbit IgG |
| WDR82 (D2I3B) Rabbit Monoclonal Antibody | 99715 | 20 µl | 30 kDa | Rabbit IgG |
| Menin (D45B1) Rabbit Monoclonal Antibody | 6891 | 20 µl | 76 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
Storage
Background
Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 on lysine 4 (2-6). SET1A, SET1B, MLL1 and MLL2 mediate di- and tri-methylation of histone H3 Lys4 at gene promoters to facilitate transcription activation. MLL3 and MLL4 function primarily to mono-methylate histone H3 Lys4 at gene enhancers. MLL1 and MLL2 function as master regulators of both embryogenesis and hematopoiesis, and are required for proper expression of Hox genes (8-10). MLL1 is a large approximately 4000 amino acid protein that is cleaved by the Taspase 1 threonine endopeptidase to form N-terminal (MLL1-N) and C-terminal MLL1 (MLL1-C) fragments, both of which are subunits of the functional MLL1/COMPASS complex (11,12). MLL1 translocations are found in a large number of hematological malignancies, suggesting that Set1 histone methyltransferase complexes play a critical role in leukemogenesis (6). Like MLL1, MLL2 is also a large, approximately 2700 amino acid protein that is cleaved by the Taspase 1 threonine endopeptidase to form N-terminal (MLL2-N) and C-terminal (MLL2-C) fragments, both of which are subunits of the functional MLL2/COMPASS complex. MLL2 has also been implicated as a modulator of hematological malignancies (13). MLL3 and MLL4 proteins are not cleaved by Taspase 1.
Background References
- Miller, T. et al. (2001) Proc Natl Acad Sci U S A 98, 12902-7.
- Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
- Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36.
- Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
- Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
- Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.
- Yokoyama, A. et al. (2004) Mol Cell Biol 24, 5639-49.
- Eissenberg, J.C. and Shilatifard, A. (2010) Dev Biol 339, 240-9.
- Smith, E. et al. (2011) Genes Dev 25, 661-72.
- Denissov, S. et al. (2014) Development 141, 526-37.
- Takeda, S. et al. (2006) Genes Dev 20, 2397-409.
- Yokoyama, A. et al. (2002) Blood 100, 3710-8.
- Chen, Y. et al. (2017) Cancer Cell 31, 755-770.e6.
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