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2287
Shh Antibody
Primary Antibodies
Polyclonal Antibody

Shh Antibody #2287

Citations (7)

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# Product Name Applications Reactivity
  • Western Blotting
H R
No image available

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 19, 42, 45
SOURCE Rabbit

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Shh antibody detects both the precursor and mature forms of transfected Shh.

Species predicted to react based on 100% sequence homology:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His35 of human Shh. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh) and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45 kDa precursors that undergo auto-cleavage to generate a 19 kDa amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The amino-terminal peptide becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6).

  1. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87.
  2. McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114.
  3. Zhang, X.M. et al. (2001) Cell 106, 781-92.
  4. Adolphe, C. et al. (2004) Development 131, 5009-19.
  5. Pathi, S. et al. (2001) Mech Dev 106, 107-17.
  6. Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.
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