Revision 1

#69204

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SIX3 Antibody
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
30, 38, 45

Source/Isotype:
Rabbit

UniProt ID:
#O95343

Entrez-Gene Id:
6496

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

SIX3 Antibody recognizes endogenous levels of total SIX3 protein. This antibody is not predicted to cross-react with other SIX proteins, based on sequence divergence in the epitope region.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg281 of human SIX3 protein. Antibodies are purified by peptide affinity chromatography.

Background

Sine oculis homeobox (SIX) proteins belong to a family of evolutionarily conserved transcription factors discovered in Drosophila mutant screens for embryonic eye development genes (1-3). The prototypical family member (sine oculis, so) was named for eyeless embryos carrying mutations in a gene highly conserved among vertebrates, including humans (SIX1) (4). A total of six family members (SIX1-6) have been identified in vertebrates. Each SIX protein contains a homeobox nucleic acid recognition domain (HD) with a DNA-binding helix-turn-helix motif and an adjacent SIX domain, which may be involved in regulating protein-protein interactions (5). In addition to their critical functions during embryonic organogenesis, research studies suggest that SIX proteins play additional roles in postnatal cell cycle regulation, with potentially important implications in tumorigenesis (6,7). In addition to its well-established role within gene networks regulating neural patterning and brain development (8,9), SIX3 was more recently reported to regulate postnatal pancreatic β cell maturation, in part by suppressing the expression of genes associate with fetal β cells and various non-β cell types (10,11). Dysregulated expression of SIX3 has also been linked with tumor development. For example, in a study of cell free DNA isolated from plasma, SIX3 was identified as a differentially methylated gene that was diagnostically relevant for patients with pancreatic ductal adenocarcinoma (12). This finding was consistent with earlier reports supporting a role for SIX3 as a tumor suppressor (13-15).

Background References

  1. Kumar, J.P. (2009) Cell Mol Life Sci 66, 565-83.
  2. Fischbach, K.F. and Technau, G. (1984) Dev Biol 104, 219-39.
  3. Fischbach, K.F. and Heisenberg, M. (1981) Proc Natl Acad Sci U S A 78, 1105-9.
  4. Boucher, C.A. et al. (1996) Genomics 33, 140-2.
  5. Pignoni, F. et al. (1997) Cell 91, 881-91.
  6. Ford, H.L. et al. (1998) Proc Natl Acad Sci U S A 95, 12608-13.
  7. Coletta, R.D. et al. (2004) Proc Natl Acad Sci U S A 101, 6478-83.
  8. Wallis, D.E. and Muenke, M. (1999) Mol Genet Metab 68, 126-38.
  9. Oliver, G. and Gruss, P. (1997) Trends Neurosci 20, 415-21.
  10. Arda, H.E. et al. (2016) Cell Metab 23, 909-20.
  11. Bevacqua, R.J. et al. (2021) Genes Dev 35, 234-249.
  12. Li, S. et al. (2020) Front Genet 11, 596078.
  13. Yu, Z. et al. (2017) J Hematol Oncol 10, 115.
  14. Zhang, B. et al. (2017) J Neurooncol 133, 509-518.
  15. Zheng, Y. et al. (2018) Theranostics 8, 972-989.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human

Trademarks and Patents

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XP is a registered trademark of Cell Signaling Technology, Inc.

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Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.