Revision 3

#54984

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, W-S, IP

Reactivity:
H M R Mk

Sensitivity:
Endogenous

MW (kDa):
78

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q9Y5X1

Entrez-Gene Id:
51429

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

SNX9 (F9G1M) Rabbit Monoclonal Antibody recognizes endogenous levels of total SNX9 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SNX9 protein.

Background

Sorting nexins (SNXs) are a family of cytoplasmic proteins characterized by the presence of a phosphatidylinositol 3-phosphate (PI3P) binding phox (PX) domain. This binding occurs mainly in the early endosome and allows for trafficking of the bound protein to either a degradative or recycling pathway (1). Sorting nexin family member 9 (SNX9) is a multifunctional scaffolding protein that plays an important role in clathrin-mediated endocytosis (CME) (2). In CME, SNX9 binds to the protein clathrin at the plasma membrane and recruits the GTPase dynamin to facilitate membrane fission and vesicle release (3). SNX9 also recruits the protein N-WASP to the cell membrane, a key regulator of actin dynamics (4). SNX9’s function in actin assembly contributes to cancer progression, primarily by affecting cell invasion and metastasis. For instance, SNX9 has been shown to promote metastasis in breast cancer through its interaction with N-WASP and RhoA-ROCK (5). Interestingly, SNX9 has also been shown to have reduced expression in primary tumors relative to healthy tissue (6). In this context, SNX9 downregulation promotes the formation of actin-rich structures known as invadopodia, which are required for cancer cells to invade through the basement membrane (6). SNX9 is also recruited to immunological synapses during T cell activation, where it complexes with N-WASP, p85, and CD28 to internalize CD28 via CME (7,8). Furthermore, targeted knockout of SNX9 has been shown to alleviate CD8+ T cell exhaustion, suggesting its use as a therapeutic target in cancer immunotherapies (9).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting W-S: Simple Western™ IP: Immunoprecipitation

Cross-Reactivity Key

H: Human M: Mouse R: Rat Mk: Monkey

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using SNX9 (F9G1M) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of SNX9 protein in SU-DHL-4 cells is consistent with the predicted expression pattern. 
Western Blotting Image 1: SNX9 (F9G1M) Rabbit Monoclonal Antibody
Western blot analysis of extracts from HCT 116 cells, transfected with control siRNA (-) or SNX9 siRNA (+), using SNX9 (F9G1M) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 2: SNX9 (F9G1M) Rabbit Monoclonal Antibody
Simple WesternTM analysis of lysates (1 mg/ml) from C6 cells using SNX9 (F9G1M) Rabbit mAb #54984. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the JessTM Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230kDa.
Western Blotting Image 1: SNX9 (F9G1M) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunoprecipitation of SNX9 protein from HCT 116 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SNX9 (F9G1M) Rabbit mAb. Western blot analysis was performed using SNX9 (F9G1M) Rabbit mAb. Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) #93702 was used as a secondary antibody.
Immunoprecipitation Image 1: SNX9 (F9G1M) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.