WB, IP
H M R
Endogenous
22
Rabbit
#O14508
8835
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxyl terminus of human SOCS2. Antibodies are purified by peptide affinity chromatography.
Background
The suppressor of cytokine signaling (SOCS) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway (1-3). The SOCS family consists of at least 8 members including the originally identified cytokine-inducible SH2-containing protein (CIS1), as well as SOCS1-7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses.
Activity of SOCS2 has been predominantly linked to growth hormone (GH) and insulin-like growth factor 1 (IGF-1) signaling but may also contribute to several biological processes including metabolism, bone formation, neuronal development, cancer, infection and other cytokine-dependent pathways (5). SOCS2 is widely expressed in adult and fetal tissues and is induced upon cytokine treatment (5,6). A number of studies suggest that SOCS2 can have either a positive or negative effect on GH/cytokine signaling (7-10). Mice deficient in SOCS2 grow signficantly larger than normal littermates (8). SOCS2 binds to tyrosine-phosphorylated GH and IGF-1 receptors via its SH2 domain, suppressing their signaling (6,9). In addition, the SOCS box of SOCS2 binds to Elongin B and C leading to activity as a ubiquitin ligase, promoting the degradation of the receptors as well as other SOCS family members (11-13).
- Alexander, W.S. et al. (1999) J Leukoc Biol 66, 588-92.
- Chen, X.P. et al. (2000) Immunity 13, 287-90.
- Hilton, D.J. et al. (1998) Proc Natl Acad Sci USA 95, 114-9.
- Rico-Bautista, E. et al. (2006) Cytokine Growth Factor Rev 17, 431-9.
- Starr, R. et al. (1997) Nature 387, 917-21.
- Dey, B.R. et al. (1998) J Biol Chem 273, 24095-101.
- Favre, H. et al. (1999) FEBS Lett 453, 63-6.
- Metcalf, D. et al. (2000) Nature 405, 1069-73.
- Greenhalgh, C.J. et al. (2002) J Biol Chem 277, 40181-4.
- Greenhalgh, C.J. et al. (2005) J Clin Invest 115, 397-406.
- Tannahill, G.M. et al. (2005) Mol Cell Biol 25, 9115-26.
- Bullock, A.N. et al. (2006) Proc Natl Acad Sci U S A 103, 7637-42.
- Piessevaux, J. et al. (2006) J Biol Chem 281, 32953-66.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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