WB, IF-F
M R
Endogenous
40
Rabbit
#O00570
6656
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:100 - 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse, Rat
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy terminus of human Sox1. Antibodies are purified by Protein A and peptide affinity chromatography.
Background
Sox (Sry-related box) genes encode a family of transcription factors that play a myriad of roles in developmental processes (1). Sox1 and Sox2 are the earliest markers that identify neuroectodermal tissue, and these markers, together with Sox9 collectively mark neuronal stem cells (NSC's) that are present in neurogenically active areas of the mature rodent brain (2). Sox1 has been found in the walls of the lateral ventricles and the dentate gyrus, as well as in inactive areas such as the cerebellum where it marks a population of cells in the Purkinje layer known as the Bergmann glia (2). Sox1 is thought to maintain the cell cycle and promote self renewal in NSCs, but also functions in other cell types to promote differentiation (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-F: Immunofluorescence (Frozen)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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