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96443
PhosphoPlus® Sox2 (Ser250/Ser251) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® Sox2 (Ser250/Ser251) Antibody Duet #96443

Citations (0)
Western blot analysis of extracts from NTERA2 and NCCIT cells using Sox2 (D6D9) XP® Rabbit mAb.
Western blot analysis of extracts from NCCIT cells either untreated (-) or treated with lambda phosphatase (+) using Phospho-Sox2 (Ser250/Ser251) (A2I7G) Rabbit mAb.
Confocal immunofluorescent analysis of NTERA2 (left) and HeLa (right) cells using Sox2 (D6D9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using Sox2 (D6D9) XP® Rabbit mAb.
To Purchase # 96443
Cat. # Size Qty. Price
96443S
1 Kit

Product Includes Quantity Reactivity MW(kDa) Isotype
Phospho-Sox2 (Ser250/Ser251) (A2I7G) Rabbit mAb 92186 100 µl H M 35 Rabbit IgG
Sox2 (D6D9) XP® Rabbit mAb 3579 100 µl H 35 Rabbit 

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been carefully selected to provide superior performance in specified applications.

Background

Embryonic stem cells (ESC) derived from the inner cell mass of the blastocyst are unique in their pluripotent capacity and potential for self-renewal (1). Research studies demonstrate that a set of transcription factors that includes Oct-4, Sox2, and Nanog forms a transcriptional network that maintains cells in a pluripotent state (2,3). Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development (4,5). siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (6). Induced overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram both mouse and human somatic cells to a pluripotent state (7,8). Additional evidence demonstrates that Sox2 is also present in adult multipotent progenitors that give rise to some adult epithelial tissues, including several glands, the glandular stomach, testes, and cervix. Sox2 is thought to regulate target gene expression important for survival and regeneration of these tissues (9).

Phosphorylation on these and other sites on Sox2 have been observed in pluripotent cells as they undergo differentiation, although the mechanism and consequence of this potential regulation is not clear (10).

Pathways

Explore pathways related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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