WB
H
Endogenous
40
Rabbit
#Q9BUD6
10417
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu273 of human SPON2 protein. Antibodies are purified by peptide affinity chromatography.
Background
SPON2 (spondin 2, mindin) is an extracellular matrix protein belonging to the mindin/F-spondin family (1). The protein has two amino terminal follistatin-like (FS) domains and a carboxy terminal thrombospondin type 1 repeat (TSR) domain. The FS domains mediate interactions between SPON2 and integrins, whereas the TSR domain contributes to the role of SPON2 as a pathogen pattern recognition molecule (2). In that capacity, SPON2 plays an important role in initiation of the innate immune response. It functions as a pattern recognition molecule within the extracellular matrix, where it binds to microbial pathogens and presents them to immune cells (3). Interactions between SPON2 and integrins are also critical for inflammatory cell recruitment and T cell priming (4,5). In addition to its role in innate immunity, SPON2 has also been shown to play a role in hippocampal neuronal outgrowth, cancer metastasis, and diabetic nephropathy (6-8).
- Feinstein, Y. and Klar, A. (2004) Int J Biochem Cell Biol 36, 975-80.
- Li, Y. et al. (2009) EMBO J 28, 286-97.
- He, Y.W. et al. (2004) Nat Immunol 5, 88-97.
- Jia, W. et al. (2005) Blood 106, 3854-9.
- Li, H. et al. (2006) EMBO J 25, 4097-107.
- Feinstein, Y. et al. (1999) Development 126, 3637-48.
- Lu, H. et al. (2020) J Cell Mol Med 24, 98-113.
- Kahvecioglu, S. et al. (2015) Nephrology (Carlton) 20, 721-726.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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