WB, IP, ChIP
H Mk
Endogenous
400
Rabbit IgG
#Q6ZRS2
10847
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro911 of human SRCAP protein.
Background
Snf2-Related CBP Activator Protein (SRCAP) is an ATPase that exists in a large complex that activates transcription by enhancing CBP activity and by depositing H2A.Z into nucleosomes (1-3). SRCAP can enhance nuclear receptor function along with CARM-1 and GRIP-1 (4). In prostate cancer, SRCAP associates with androgen receptor to control prostate specific antigen expression and increase cell growth (5). During double-stranded break repair, SRCAP is required for DNA-end resection and RAD51 recruitment (6). Mutations in SRCAP result in a developmental disorder called Floating-Harbor syndrome (7,8). SRCAP also plays a role in viral infection as it has been shown to interact with various viral proteins (9,10).
- Johnston, H. et al. (1999) J Biol Chem 274, 16370-6.
- Monroy, M.A. et al. (2001) J Biol Chem 276, 40721-6.
- Ruhl, D.D. et al. (2006) Biochemistry 45, 5671-7.
- Monroy, M.A. et al. (2003) Mol Endocrinol 17, 2519-28.
- Slupianek, A. et al. (2010) J Cell Physiol 224, 369-75.
- Dong, S. et al. (2014) Curr Biol 24, 2097-2110.
- Hood, R.L. et al. (2012) Am J Hum Genet 90, 308-13.
- Le Goff, C. et al. (2013) Hum Mutat 34, 88-92.
- Ghosh, A.K. et al. (2000) J Biol Chem 275, 7184-8.
- Xu, X. et al. (2001) J Virol 75, 10033-40.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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