WB, W-S
H M R
Endogenous
35
Rabbit IgG
#Q16629
6432
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro86 of human SRSF7 protein.
Background
The serine/arginine-rich family of splicing factors are crucial regulators in precursor RNA splicing, a process that is associated with maintenance of genome stability, regulation of mRNA export and stability, and translation (1).
Serine/arginine-rich splicing factor 7 (SRSF7/SFRS7), also known as splicing factor 9G8, is overexpressed in colon and lung cancer, and regulates proliferation and apoptosis through the splicing of Fas mRNA in human colon and lung cancer cells (2). In human colon cancer cells, SRSF7 knockdown increased levels of the CDK inhibitor p21 Waf1/Cip1 in a p53-independent manner (3). SRSF7 also regulates age-dependent splicing in mice (4) and induces expression of cellular senescence markers in response to DNA damage by generating an alternatively spliced form of p53, p53β (5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting W-S: Simple Western™
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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