WB
H
Endogenous
80, 95
Rabbit
#Q9Y5Y6
6768
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn280 of human ST14/Matriptase protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Suppressor of tumorigenicity 14 protein (ST14), also known as matriptase, is a type II transmembrane protease (1,2). It is highly expressed in epithelia cells and important for the differentiation and homeostasis of epithelium (3). ST14 is synthesized as a single-chain precursor, and cleaved first at Gly149 in endoplasmic reticulum or Golgi. The resulting N-terminal and C-terminal fragments are non-covalently associated, and interact with hepatocyte growth factor activator inhibitor 1 (HAI-1), which facilitates the transportation of ST14 to the plasma membrane. Cell surface ST14 can be activated by autocleavage at Arg614 though an incompletely understood mechanism. HAI-1 inhibits activated ST14, and HAI-1 and ST14 complex can be shed from cell surface (4,5). In different context, ST14 has been reported either as a tumor suppressor or as a tumor promoter (6-8)
- Kim, M.G. et al. (1999) Immunogenetics 49, 420-8.
- Lin, C.Y. et al. (1999) J Biol Chem 274, 18231-6.
- Oberst, M.D. et al. (2003) J Histochem Cytochem 51, 1017-25.
- List, K. et al. Mol Med 12, 1-7.
- Bugge, T.H. et al. (2007) Front Biosci 12, 5060-70.
- Kosa, P. et al. (2012) Oncogene 31, 3679-95.
- Sales, K.U. et al. (2015) Oncogene 34, 4664-72.
- Ko, C.J. et al. (2015) Cancer Res 75, 2949-60.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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