Revision 1

#93130Store at -20C

1 Kit

(6 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
Stat1 (D1K9Y) Rabbit mAb 14994 20 µl 84, 91 kDa Rabbit IgG
Stat2 (D9J7L) Rabbit mAb 72604 20 µl 97, 113 kDa Rabbit IgG
Stat3 (D1B2J) Rabbit mAb 30835 20 µl 79, 86 kDa Rabbit IgG
Stat4 (C46B10) Rabbit mAb 2653 20 µl 81 kDa Rabbit 
Stat5 (D2O6Y) Rabbit mAb 94205 20 µl 90 kDa Rabbit IgG
Stat6 (D3H4) Rabbit mAb 5397 20 µl 110 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

Stat Antibody Sampler Kit II provides an economical means to examine the complete Stat family: Stat1-6. The kit contains enough a primary antibody to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Jaks (Janus Kinases) and Stats (Signal Transducers and Activators of Transcription) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats (2,3), and by the combinatorial coupling of various Stat members to different receptors. Serine phosphorylation in the carboxy-terminal transcriptional activation domain has been shown to regulate the function of Stat1, -2, -3, -4 and -5 (1). Phosphorylation of Stat3 at Ser727 via MAPK or mTOR pathways is required for optimal transcriptional activation in response to growth factors and cytokines including IFN-gamma and CNTF (4,5). Jak/Stat pathways also play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses and stem cell differentiation (6-11).

  1. Darnell Jr., J. et al. (1994) Science 264, 1415-1421.
  2. Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
  3. Caldenhoven, E. et al. (1996) J. Biol. Chem. 271, 13221-13227.
  4. Wen, Z. et al. (1995) Cell 82, 241-250.
  5. Yokogami, K. et al. (2000) Curr. Biol. 10, 47-50.
  6. Lim, C.P. and Cao, X. (1999) J. Biol. Chem. 274, 31055-31061.
  7. Bromberg, J. F. et al. (1999) Cell 98, 295-303.
  8. Su, L. et al. (1999) J. Biol. Chem. 274, 31770-31774.
  9. Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.
  10. Cattaneo, E. et al. (1999) Trends Neurosci. 22, 365-369.
  11. Frank, D.A. (1999) Mol. Med. 5, 432-456.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    For Research Use Only. Not for Use in Diagnostic Procedures.
    Revision 1
    #93130

    Stat Antibody Sampler Kit II

    Stat Antibody Sampler Kit II: Image 1 Expand Image
    CUT&RUN was performed with HT-1080 cells treated with (hIFN-γ) #8901 (50 ng/ml, 30 min) and Stat1 (D1K9Y) Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human AIM2 promoter primers, human FZD5 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    Stat Antibody Sampler Kit II: Image 2 Expand Image
    Simple Western™ analysis of lysates (0.1 mg/mL) from MCF-7 cells using Stat1 (D1K9Y) Rabbit mAb #14994. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ ​​​​​​​ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
    Stat Antibody Sampler Kit II: Image 3 Expand Image
    CUT&RUN was performed with HT-1080 cells and Stat1 (D1K9Y) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA library was prepared using DNA Library Prep Kit for Illumina (ChIP-seq, CUT&RUN) #56795. The figure shows binding across FZD5 gene, a known target gene of Stat1 (see additional figure containing CUT&RUN-qPCR data).
    Stat Antibody Sampler Kit II: Image 4 Expand Image
    CUT&RUN was performed with HT-1080 cells and Stat1 (D1K9Y) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 2 (upper), including FZD5 gene (lower), a known target gene of Stat1 (see additional figure containing CUT&RUN-qPCR data).
    Stat Antibody Sampler Kit II: Image 5 Expand Image

    Simple Western™ analysis of lysates (0.1 mg/mL) from Jurkat cells using Stat6 (D3H4) Rabbit mAb #5397. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12 – 230 kDa separation module.

    Stat Antibody Sampler Kit II: Image 6 Expand Image
    Flow cytometric analysis of K-562 cells using Stat2 (D9J7L) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
    Stat Antibody Sampler Kit II: Image 7 Expand Image
    Simple Western™ analysis of lysates (0.1 mg/mL) from HDLM-2 cells using Stat5 (D2O6Y) Rabbit mAb #94205. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
    Stat Antibody Sampler Kit II: Image 8 Expand Image
    Simple Western™ analysis of lysates (0.1 mg/mL) from HDLM-2 cells using Stat5 (D2O6Y) Rabbit mAb #94205. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ ​​​​​​​ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
    Stat Antibody Sampler Kit II: Image 9 Expand Image
    Western blot analysis of extracts from A549 cells (lane 1) or STAT1 knock-out cells (lane 2) using Stat1 (D1K9Y) Rabbit mAb #14994 (upper), and β-actin (D6A8) Rabbit mAb #8457 (lower). The absence of signal in the STAT1 knock-out A549 cells confirms specificity of the antibody for STAT1.
    Stat Antibody Sampler Kit II: Image 10 Expand Image
    Western blot analysis of extracts from various cell lines and from rat thymus using Stat4 (C46B10) Rabbit mAb.
    Stat Antibody Sampler Kit II: Image 11 Expand Image
    Western blot analysis of extracts from various cell lines using Stat3 (D1B2J) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
    Stat Antibody Sampler Kit II: Image 12 Expand Image
    Western blot analysis of extracts from various cell lines using Stat6 (D3H4) Rabbit mAb.
    Stat Antibody Sampler Kit II: Image 13 Expand Image
    After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
    Stat Antibody Sampler Kit II: Image 14 Expand Image
    Western blot analysis of extracts from various cell lines using Stat2 (D9J7L) Rabbit mAb. KARPAS cell line source: Dr Abraham Karpas at the University of Cambridge.
    Stat Antibody Sampler Kit II: Image 15 Expand Image
    CUT&RUN was performed with U266 cells treated with Human Interferon-α1 (hIFN-α1) #8927 (10nM, 30min) and Stat2 (D9J7L) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Library was prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across GAS5 gene.
    Stat Antibody Sampler Kit II: Image 16 Expand Image
    Western blot analysis of extracts from various cell lines using Stat5 (D2O6Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
    Stat Antibody Sampler Kit II: Image 17 Expand Image
    Western blot analysis of extracts from various cell lines using Stat1 (D1K9Y) Rabbit mAb.
    Stat Antibody Sampler Kit II: Image 18 Expand Image
    Western blot analysis of extracts from ACHN, SR and Caki cell lines and from rat thymus using Stat4 (C46B10) Rabbit mAb.
    Stat Antibody Sampler Kit II: Image 19 Expand Image
    Western blot analysis of extracts from NIH/3T3 cells, mock transfected (-) or transfected with an siRNA against mouse Stat3 (mStat3 siRNA; +), using Stat3 (D1B2J) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
    Stat Antibody Sampler Kit II: Image 20 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from Ramos cells starved overnight then treated with IL-4 (100 ng/ml, 30 min) and either Stat6 (D3H4) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human DMD Intron 2 Primers #7710, human MS4A1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    Stat Antibody Sampler Kit II: Image 21 Expand Image
    Immunoprecipitation of Stat2 from KARPAS-299 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Stat2 (D9J7L) Rabbit mAb. Western blot was performed using Stat2 (D9J7L) Rabbit mAb. KARPAS cell line source: Dr Abraham Karpas at the University of Cambridge.
    Stat Antibody Sampler Kit II: Image 22 Expand Image
    CUT&RUN was performed with U266 cells treated with Human Interferon-α1 (hIFN-α1) #8927 (10nM, 30min) and Stat2 (D9J7L) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Library was prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 1 (upper), including GAS5 gene (lower).
    Stat Antibody Sampler Kit II: Image 23 Expand Image
    Western blot analysis of PC-3 cells, mock transfected (-) or transfected with constructs expressing full-length human Stat5a (+) or Stat5b (+) using Stat5 (D2O6Y) Rabbit mAb.
    Stat Antibody Sampler Kit II: Image 24 Expand Image
    Immunoprecipitation of Stat1 from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Stat1 (D1K9Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Stat1 (9H2) Mouse mAb #9176.
    Stat Antibody Sampler Kit II: Image 25 Expand Image
    Immunoprecipitation of Stat4 protein from NK-92 cell extracts. Lane 1 is 10% input, lane 2 is beads only, lane 3 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 4 is Stat4 (C46B10) Rabbit mAb. Western blot analysis was performed using Stat4 (C46B10) Rabbit mAb.
    Stat Antibody Sampler Kit II: Image 26 Expand Image
    Immunoprecipitation of Stat3 from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Stat3 (D1B2J) Rabbit mAb. Western blot was performed using Stat3 (D1B2J) Rabbit mAb. A conformation-specific secondary antibody was used to avoid reactivity with IgG.
    Stat Antibody Sampler Kit II: Image 27 Expand Image
    Confocal immunofluorescent analysis of A-431 cells, serum starved (left) or treated with IFNα (1000 U/ml for 30 mins; right) using Stat2 (D9J7L) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Stat Antibody Sampler Kit II: Image 28 Expand Image
    CUT&RUN was performed with U266 cells treated with Human Interferon-α1 (hIFN-α1) #8927 (10nM, 30min) and either Stat2 (D9J7L) Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human SIN3A promoter primers and human ITM2A upstream primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    Stat Antibody Sampler Kit II: Image 29 Expand Image
    Immunoprecipitation of Stat5 from K-562 cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Stat5 (D2O6Y) Rabbit mAb. Western blot was performed using Stat5 (D2O6Y) Rabbit mAb.
    Stat Antibody Sampler Kit II: Image 30 Expand Image
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Stat1 (D1K9Y) Rabbit mAb.
    Stat Antibody Sampler Kit II: Image 31 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from NK-92 cells starved of IL-2 overnight then treated with IL-12 (10 ng/ml) for 4 hr and Stat4 (C46B10) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across IRF1, a known target gene of Stat4 (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product datasheet.
    Stat Antibody Sampler Kit II: Image 32 Expand Image
    Immunohistochemical analysis of paraffin-embedded HeLa cell pellet (left, positive) or PC-3 cell pellet (right, negative) using Stat3 (D1B2J) Rabbit mAb.
    Stat Antibody Sampler Kit II: Image 33 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from BaF3 cells starved of IL-3 for 6 hours followed by induction with IL-3 for 45 minutes, and either Stat5 (D2O6Y) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse CIS Intron 1 Primers #5131, mouse SOCS-3 promoter primers, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    Stat Antibody Sampler Kit II: Image 34 Expand Image
    Immunohistochemical analysis of paraffin-embedded human lymphoma using Stat1 (D1K9Y) Rabbit mAb.
    Stat Antibody Sampler Kit II: Image 35 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from NK-92 cells starved of IL-2 overnight then treated with IL-12 (10 ng/ml) for 4 hr and Stat4 (C46B10) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across chromosome 5 (upper), including IRF1 (lower), a known target gene of Stat4 (see additional figure containing ChIP-qPCR data).
    Stat Antibody Sampler Kit II: Image 36 Expand Image
    Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Stat3 (D1B2J) Rabbit mAb.
    Stat Antibody Sampler Kit II: Image 37 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from U266 cells treated with Human Interferon-α (IFN-α) #9906 (10nM) for 30 min and Stat2 (D9J7L) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across USP18, a known target gene of Stat2 (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product datasheet.
    Stat Antibody Sampler Kit II: Image 38 Expand Image
    Confocal immunofluorescent analysis of HeLa cells, serum-starved overnight (left) or treated with Human Interferon-α1 (hIFN-α1) #8927 (1,000 units/ml, 30 min; right), using Stat1 (D1K9Y) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).
    Stat Antibody Sampler Kit II: Image 39 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from NK-92 cells starved of IL-2 overnight then treated with IL-12 (10 ng/ml) for 4 hr and either Stat4 (C46B10) Rabbit mAb #2653 or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, human PRF1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    Stat Antibody Sampler Kit II: Image 40 Expand Image
    Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Stat3 (D1B2J) Rabbit mAb.
    Stat Antibody Sampler Kit II: Image 41 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from U266 cells treated with Human Interferon-α (IFN-α) #9906 (10nM) for 30 min and Stat2 (D9J7L) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across chromosome 22 (upper), including USP18 (lower), a known target gene of Stat2 (see additional figure containing ChIP-qPCR data).
    Stat Antibody Sampler Kit II: Image 42 Expand Image
    Flow cytometric analysis of ACHN cells using Stat1 (D1K9Y) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
    Stat Antibody Sampler Kit II: Image 43 Expand Image
    Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Stat3 (D1B2J) Rabbit mAb.
    Stat Antibody Sampler Kit II: Image 44 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from U266 cells treated with Human Interferon-α (IFN-α) #9906 (100 ng/ml) for 30 min, and either Stat2 (D9J7L) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human USP18 promoter primers, SimpleChIP® Human WARS Intron 1 Primers #30101, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    Stat Antibody Sampler Kit II: Image 45 Expand Image
    Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Stat3 (D1B2J) Rabbit mAb.
    Stat Antibody Sampler Kit II: Image 46 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from HT-1080 cells treated with Human Interferon-γ (hIFN-γ) #8901 (50 ng/ml, 30 min) and either Stat1 (D1K9Y) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    Stat Antibody Sampler Kit II: Image 47 Expand Image
    Confocal immunofluorescent analysis of HeLa cells (Stat3 positive), serum-starved (left) or treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/mL, 20 min; center), or PC-3 cells (Stat3 negative) treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/mL, 20 min; right), using Stat3 (D1B2J) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).
    Stat Antibody Sampler Kit II: Image 48 Expand Image
    Western blot analysis of extracts from HeLa control cells (lane 1) or Stat3 knockout cells (lane 2) using Stat3 (D1B2J) Rabbit mAb #30835 (upper) or β-actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the Stat3 knockout cells confirms the specificity of the antibody for Stat3.
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    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    For Research Use Only. Not for Use in Diagnostic Procedures.