WB, IP
H R
Endogenous
30
Rabbit IgG
#Q9UHE8
26872
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human STEAP1 protein.
Background
Six transmembrane epithelial antigen of the prostate 1 (STEAP1) is a transmembrane protein abundantly expressed in normal prostate epithelial cells (1). It is a metalloreductase that reduces Fe3+ to Fe2+ and Cu2+ to Cu1+ (2). STEAP1 is also reportedly overexpressed in multiple tumor types, and has been used as a cell surface biomarker for prostate cancer and Ewing's sarcoma (3-6). Peptides derived from STEAP1 protein have been shown to induce an antigen-specific cytotoxic T lymphocyte (CTL) response that can kill tumor cells expressing STEAP1 (7). These finding are the basis for research studies examining the efficacy of antibody-drug conjugates (ADCs) targeting STEAP1 (8,9).
- Hubert, R.S. et al. (1999) Proc Natl Acad Sci U S A 96, 14523-8.
- Ohgami, R.S. et al. (2006) Blood 108, 1388-94.
- Grunewald, T.G. et al. (2012) Mol Cancer Res 10, 52-65.
- Grunewald, T.G. et al. (2012) Ann Oncol 23, 2185-90.
- Ihlaseh-Catalano, S.M. et al. (2013) Histopathology 63, 678-85.
- Gomes, I.M. et al. (2014) Urol Oncol 32, 53.e23-9.
- Rodeberg, D.A. et al. (2005) Clin Cancer Res 11, 4545-52.
- Williams, S.P. et al. (2016) Oncotarget 7, 25103-12.
- Sukumaran, S. et al. (2016) AAPS J , .
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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