WB, IP, IF-IC, ChIP, ChIP-seq
H M R
Endogenous
50
Rabbit IgG
#Q13285
2516
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:100 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Bovine
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu184 of human STF-1 protein.
Background
The orphan nuclear receptor, steroidogenic factor 1 (STF-1, also called Ad4BP), is encoded by the NR5A1 gene and plays an instrumental role in directing the transcriptional control of steroidogenesis (1). Initially identified as a tissue-specific transcriptional regulator of cytochrome P450 steroid hydroxylases, research studies of both global (2) and tissue-specific knockout mice (3-6) have demonstrated that STF-1 is required for the development of adrenal glands, gonads, ventromedial hypothalamus, and for the proper functioning of pituitary gonadotropes. Indeed, humans with mutations that render STF-1 transcriptionally inactive can present with testicular failure, ovarian failure, and adrenal insufficiency (7,8). Furthermore, dysregulation of STF-1 has been linked to diseases such as endometriosis (9) and adrenocortical carcinoma (10).
Like other nuclear hormone receptors, STF-1 has a modular domain structure composed of an amino-terminal zinc finger DNA-binding domain, a ligand-binding domain, a carboxy-terminal AF-2 activation domain, and a hinge region with AF-1-like activation activity. STF-1 also contains a fushi tarazu factor 1 box, which functions as an accessory DNA binding domain (11). STF-1 is primarily phosphorylated at Ser203, which is thought to enhance its transcriptional activity by promoting complex formation with transcriptional cofactors (12). In addition to phosphorylation at Ser203, STF-1 is subject to SUMO conjugation and acetylation at ε-amino groups of target lysine residues. Whereas SUMOylation represses STF-1 function (13,14), acetylation enhances its transcriptional activity (15).
- Parker, K.L. and Schimmer, B.P. (1997) Endocr Rev 18, 361-77.
- Luo, X. et al. (1994) Cell 77, 481-90.
- Zhao, L. et al. (2001) Development 128, 147-54.
- Jeyasuria, P. et al. (2004) Mol Endocrinol 18, 1610-9.
- Pelusi, C. et al. (2008) Biol Reprod 79, 1074-83.
- Zhao, L. et al. (2008) Mol Endocrinol 22, 1403-15.
- Achermann, J.C. et al. (1999) Nat Genet 22, 125-6.
- Lourenço, D. et al. (2009) N Engl J Med 360, 1200-10.
- Bulun, S.E. et al. (2009) Mol Cell Endocrinol 300, 104-8.
- Figueiredo, B.C. et al. (2005) J Clin Endocrinol Metab 90, 615-9.
- Little, T.H. et al. (2006) Mol Endocrinol 20, 831-43.
- Hammer, G.D. et al. (1999) Mol Cell 3, 521-6.
- Chen, W.Y. et al. (2004) J Biol Chem 279, 38730-5.
- Lee, F.Y. et al. (2011) Dev Cell 21, 315-27.
- Chen, W.Y. et al. (2005) Mol Cell Biol 25, 10442-53.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) ChIP: Chromatin IP ChIP-seq: Chromatin IP-seq
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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