| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| G3BP1 (E9G1M) XP® Rabbit mAb | 61559 | 20 µl | 68 kDa | Rabbit IgG |
| G3BP2 Antibody | 31799 | 20 µl | 60, 70 kDa | Rabbit |
| TIAR (D32D3) XP® Rabbit mAb | 8509 | 20 µl | 42 kDa | Rabbit IgG |
| FMRP (D14F4) Rabbit mAb | 7104 | 20 µl | 80 kDa | Rabbit IgG |
| UBAP2L (E5X4E) Rabbit mAb | 40199 | 20 µl | 150, 160 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Stress Granule Marker IF Antibody Sampler Kit provides an economical means of evaluating total levels of stress granule-associated proteins. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
Stress granules (SGs) are cytoplasmic condensates that form at sites of stalled mRNA translation in response to various cellular stressors. SGs are composed of translationally inactive 48S preinitiation complexes (PICs), untranslated mRNA, and a complex mixture of RNA-binding proteins (RBPs). Two key mediators of SG assembly are the RBPs Ras-GTPase-activating protein-binding proteins 1 and 2 (G3BP1/2), which bind to excess mRNA and recruit additional SG-associated proteins. Overexpression of either G3BP1 or G3BP2 is enough to induce SG formation even in the absence of stress, and reduced levels of G3BP1 or G3BP2 protein severely inhibit SG formation, highlighting their crucial role in this process (1-4). Additional SG-associated proteins include TIA-1-related protein (TIAR), fragile X mental retardation protein (FMRP), and ubiquitin-associated protein 2-like (UBAP2L). TIAR is a member of the RNA-recognition motif (RRM) family of RBPs (5,6). In response to cellular stress, TIAR associates with G3BP1/2 and its family member TIA-1 to form SG condensates (7,8). The two major isoforms of TIAR are the products of alternative mRNA splicing (9,10). FMRP (also known as FMR1) and its two autosomal homologs (FXR1 and FXR2) all bind RNA and play a role in the pathogenesis of fragile X syndrome (11-13). Each of these related proteins can associate with one another as well as form homodimers (13). FMRP can act as a translation regulator and is a component of RNAi effector complexes (RISC), suggesting a role in gene silencing (14). In addition, FMRP, FXR1, and FXR2 are components of SGs and have been implicated in the translational regulation of mRNAs (15). UBAP2L is a ubiquitous and highly conserved protein containing an N-terminal ubiquitin-associated (UBA) domain involved in the ubiquitin-proteasome system (UPS) and aggregate formation induced by proteasome inhibitors (16). It can also interact with the Polycomb group protein BMI1 to form a Polycomb subcomplex and regulate hematopoietic stem cell activity (17). UBAP2L is essential for the formation of SGs, and some studies suggest that arginine methylation by PRMT1 inhibits UBAP2L interaction with SG elements and overall SG assembly (18-20).
- Tourrière, H. et al. (2003) J Cell Biol 160, 823-31.
- Matsuki, H. et al. (2013) Genes Cells 18, 135-46.
- Kedersha, N. et al. (2016) J Cell Biol 212, 845-60.
- Yang, P. et al. (2020) Cell 181, 325-345.e28.
- Tian, Q. et al. (1991) Cell 67, 629-39.
- Kawakami, A. et al. (1992) Proc Natl Acad Sci USA 89, 8681-5.
- Anderson, P. and Kedersha, N. (2002) Cell Stress Chaperones 7, 213-21.
- Anderson, P. and Kedersha, N. (2002) J Cell Sci 115, 3227-34.
- Kawakami, A. et al. (1994) J Immunol 152, 4937-45.
- Beck, A.R. et al. (1996) Nucleic Acids Res 24, 3829-35.
- Verkerk, A.J. et al. (1991) Cell 65, 905-14.
- Siomi, M.C. et al. (1995) EMBO J 14, 2401-8.
- Zhang, Y. et al. (1995) EMBO J 14, 5358-66.
- Caudy, A.A. et al. (2002) Genes Dev 16, 2491-6.
- Linder, B. et al. (2008) Hum Mol Genet 17, 3236-46.
- Wilde, I.B. et al. (2011) J Proteome Res 10, 1062-72.
- Bordeleau, M.E. et al. (2014) Blood 124, 2362-9.
- Markmiller, S. et al. (2018) Cell 172, 590-604.e13.
- Cirillo, L. et al. (2020) Curr Biol 30, 698-707.e6.
- Huang, C. et al. (2020) Cell Death Differ 27, 227-241.
Background References
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
