WB
H M R
Endogenous
70, 74
Rabbit IgG
#Q9UH65
23075
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp536 of human SWAP70 protein.
Background
SWAP70 is a Rac family guanine nucleotide exchange factor (GEF) (1). It is highly expressed by activated B cells. Following B cell stimulation, SWAP70 has been observed to translocate from the cytoplasm to the nucleus, where it plays a role in class switching, as well as to the membrane, where it associates with the B cell receptor (2,3). SWAP70 also plays a role in migration of B cells and other immune cell types including dendritic cells, eosinophils, and mast cells (4-7). Mice deficient in both SWAP70 and a related protein, DEF6, develop lupus-like autoimmunity due to misregulation of IRF4 in B cells and T cells leading to increased IL21 production and responsiveness (8).
- Shinohara, M. et al. (2002) Nature 416, 759-63.
- Borggrefe, T. et al. (1998) J Biol Chem 273, 17025-35.
- Masat, L. et al. (2000) Proc Natl Acad Sci U S A 97, 2180-4.
- Sivalenka, R.R. and Jessberger, R. (2004) Mol Cell Biol 24, 10277-88.
- Pearce, G. et al. (2006) Nat Immunol 7, 827-34.
- Ocaña-Morgner, C. et al. (2011) J Immunol 186, 5345-55.
- Bahaie, N.S. et al. (2012) J Immunol 188, 1479-90.
- Biswas, P.S. et al. (2012) J Exp Med 209, 581-96.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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