WB
H M R Mk
Endogenous
52
Rabbit
#Q96A49
94056
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys110 of human SYAP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Synapse-associated protein 1 (SYAP1) was originally described by its similarity to the Drosophila synapse-associated SAP47 protein (1). Subsequent research using a yeast two-hybrid system described the protein as a BSD domain–containing signal transducer and Akt interactor (BSTA) based on protein structure and function. The ubiquitously expressed BSTA protein contains a central BSD domain that may play a role in mediating interaction between the BSTA protein and the serine/threonine kinase Akt1 (2). Research studies support a model of Akt1 activation that involves interactions between the BSTA protein and both Akt1 and the mTORC2 kinase complex, followed by phosphorylation of both BSTA and Akt1 by mTORC2. This series of interactions and phosphorylation events is thought to result in phosphorylation of Akt1 at Ser473 and Akt1 kinase activation (2). The BSTA mediated phosphorylation of Akt1 may promote adipocyte differentiation by suppressing the expression of the transcription factor FoxC2 (2). Additional studies show that the estrogen receptor antagonist tamoxifen can regulate the expression of BSTA in some breast cancer cells, suggesting a possible role for BSTA in pathways related to response to tamoxifen and other chemopreventative agents (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
