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8630
Synaptic Vesicle Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Synaptic Vesicle Antibody Sampler Kit #8630

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Simple Western™ analysis of lysates (0.1 mg/mL) from rat brain using Munc18-1 (D4O6V) Rabbit mAb #13414. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Western blot analysis of extracts from mouse brain, rat brain, and human cortex using Munc18-1 (D4O6V) Rabbit mAb.
Western blot analysis of extracts from various cell lines using Syntaxin 6 (C34B2) Rabbit mAb.
Western blot analysis of extracts from various cell types using NSF (D31C7) XP® Rabbit mAb.
Western blot analysis of extracts from mouse and rat brain using SNAP25 (D9A12) Rabbit mAb.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Confocal immunofluorescent analysis of MCF7 cells using Syntaxin 6 (C34B2) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 (fluorescent DNA dye).
Confocal immunofluorescent analysis of mouse brain using NSF (D31C7) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Inquiry Info.# 8630

Product Description

The Synaptic Vesicle Antibody Sampler Kit provides an economical means of evaluating proteins involved in synaptic vesicle fusion and membrane trafficking. The kit contains enough primary and secondary antibodies to perform four western miniblot experiments with each antibody.

Specificity / Sensitivity

Munc18-1 (D4O6V) Rabbit mAb recognizes endogenous levels of total Munc18-1 protein. NSF (D31C7) XP® Rabbit mAb detects endogenous levels of total NSF protein. SNAP25 (D9A12) Rabbit mAb detects endogenous levels of total SNAP25 protein. Syntaxin 6 (C34B2) Rabbit mAb detects endogenous levels of total syntaxin 6 protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr157 of human Munc18-1 protein, residues surrounding Tyr140 of mouse syntaxin 6 protein, residues surrounding Gln116 of human SNAP25 protein, or residues surrounding Leu524 of human NSF protein.

Background

Fusion of a vesicle to its target membrane is a universal process in eukaryotic cells for proper cellular organization and function. Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). SNAP25 forms a core complex with the SNARE proteins syntaxin and synaptobrevin to mediate synaptic vesicle fusion with the plasma membrane during Ca2+-dependent exocytosis (3). Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins (4,5). Munc18-1 acts as a molecular chaperone for syntaxin-1, allowing for formation of the SNARE complex at the plasma membrane (6). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (7,8). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion, and eventual recycling of the SNARE complex for subsequent membrane fusion (7,8).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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