| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Zap-70 (D1C10E) XP® Rabbit mAb | 3165 | 40 µl | 70 kDa | Rabbit IgG |
| Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb | 2717 | 40 µl | 70, 72 kDa | Rabbit IgG |
| SLP-76 Antibody | 4958 | 40 µl | 76 kDa | Rabbit |
| Phospho-LAT (Tyr220) Antibody | 3584 | 40 µl | 36, 38 kDa | Rabbit |
| LAT Antibody | 9166 | 40 µl | 36, 38 kDa | Rabbit |
| Phospho-Lck (Tyr505) Antibody | 2751 | 40 µl | 56 kDa | Rabbit |
| Lck (73A5) Rabbit mAb | 2787 | 40 µl | 56 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The T Cell Signaling Antibody Sampler Kit contains primary and secondary antibodies to perform four Western blots with each antibody.
Storage
Background
The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).
LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling (9). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (10,11). The phosphorylation of LAT at Tyr171 and 191 enables the binding of Grb2, Gads/SLP-76, PLCgamma1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (11-12).
- Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
- Iwashima, M. et al. (1994) Science 263, 1136-9.
- Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.
- Chan, A.C. et al. (1995) EMBO J 14, 2499-508.
- Williams, B.L. et al. (1999) EMBO J 18, 1832-44.
- Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.
- Wiestner, A. et al. (2003) Blood 101, 4944-51.
- Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.
- Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-83.
- Zhang, W. et al. (1998) Cell 92, 83-92.
- Paz, P.E. et al. (2001) Biochem J 356, 461-71.
- Zhang, W. et al. (2000) J Biol Chem 275, 23355-61.
Background References
Trademarks and Patents
Limited Uses
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