WB
H M Mk
Endogenous
120-140
Rabbit IgG
#Q14494
4779
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly129 of human TCF11 protein.
Background
Transcription factor 11 (TCF11) is a basic leucine zipper transcription factor. It is also referred to as Nuclear factor E2-related factor 1 (NRF1). TCF11 was initially reported to activate erythroid-specific, human globin gene expression (1). It plays an essential role during embryonic development (2). It also associates with other transcription factors, such as Jun proteins, to transcriptionally control antioxidant response element (ARE)-mediated expression in response to antioxidants and xenobiotics (3-5). TCF11 has been shown to regulate proteasomal degradation and mediate the proteasome recovery pathway after proteasome inhibition (6,7). TCF11 is ubiquitously expressed (8) and several isoforms have been reported. The 120 kDa form exists in the endoplasmic reticulum (ER) membrane under normal conditions. Upon proteasome inhibition, TCF11 translocates to the nucleus (7). The 65 kDa N-terminal-truncated form is constitutively localized to the nucleus (9,10). TCF11 protein levels are regulated by ubiquitination and proteasomal-mediated degradation (11); proteasome inhibitors stabilize TCF11.
- Caterina, J.J. et al. (1994) Nucleic Acids Res 22, 2383-91.
- Murphy, P. and Kolstø, A. (2000) Mech Dev 97, 141-8.
- Johnsen, O. et al. (1998) Nucleic Acids Res 26, 512-20.
- Venugopal, R. and Jaiswal, A.K. (1998) Oncogene 17, 3145-56.
- Kwong, M. et al. (1999) J Biol Chem 274, 37491-8.
- Radhakrishnan, S.K. et al. (2010) Mol Cell 38, 17-28.
- Steffen, J. et al. (2010) Mol Cell 40, 147-58.
- Chan, J.Y. et al. (1993) Proc Natl Acad Sci USA 90, 11371-5.
- Wang, W. and Chan, J.Y. (2006) J Biol Chem 281, 19676-87.
- Wang, W. et al. (2007) J Biol Chem 282, 24670-8.
- Chepelev, N.L. et al. (2011) PLoS One 6, e29167.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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