WB
H M R Mk
Endogenous
23
Rabbit IgG
#P13693
7178
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TCTP protein.
Background
Translationally controlled tumor protein (TCTP/p23/HRF) is a ubiquitously expressed and highly conserved protein involved in various cellular processes, such as its role as a histamine releasing factor in chronic allergic disease (1). TCTP binds tubulin in a cell cycle dependent manner and is associated with the mitotic spindle (2). In addition, TCTP interacts with the actin cytoskeleton to regulate cell shape (3). In mitosis, TCTP is phosphorylated by PLK at Ser46, decreasing microtubule stability (4,5). TCTP interacts with the small GTPase Rheb, possibly acting as a GEF, thereby activating the TORC1 pathway and controlling cell growth and proliferation (6,7). TCTP has also been shown to be involved in apoptosis and cell stress (8-11). In cultured cells, reduction in TCTP expression can cause loss of the malignant phenotype (12).
- MacDonald, S.M. et al. (1995) Science 269, 688-90.
- Gachet, Y. et al. (1999) J Cell Sci 112 ( Pt 8), 1257-71.
- Bazile, F. et al. (2009) Carcinogenesis 30, 555-65.
- Yarm, F.R. (2002) Mol Cell Biol 22, 6209-21.
- Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.
- Dong, X. et al. (2009) J Biol Chem 284, 23754-64.
- Hsu, Y.C. et al. (2007) Nature 445, 785-8.
- Susini, L. et al. (2008) Cell Death Differ 15, 1211-20.
- Bommer, U.A. et al. (2009) Oncogene [Epub ahead of print].
- Gnanasekar, M. et al. (2009) Biochem Biophys Res Commun 386, 333-7.
- Gnanasekar, M. et al. (2009) Int J Oncol 34, 1241-6.
- Tuynder, M. et al. (2002) Proc Natl Acad Sci USA 99, 14976-81.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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