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Render Timestamp:
6/9/2026, 6:04:33 AM EDT
6/9/2026, 10:04:33 AM UTC
Commit: 33ae406c5df2bbc7a7d3c17605228fbeff8ea416
XML generation date: 2026-06-05 17:33:15.969
Product last modified at: 2026-05-14T08:00:16.595Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Tec (F3M5R) Rabbit Monoclonal Antibody #89611

Filter:
  • WB
  • IP
  • F

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 74
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Tec (F3M5R) Rabbit Monoclonal Antibody recognizes endogenous levels of total Tec protein. This antibody does not cross-react with Btk, Itk, or Etk proteins.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Tec protein.

    Background

    Tec kinase belongs to a structurally related subfamily of protein tyrosine kinases (PTKs) that includes Btk, Itk (also known as Emt or Tsk), Bmx, and Txk (or Rlk) (1). The members of this subfamily possess a long amino-terminal region consisting of a pleckstrin homology (PH) domain and a Tec homology (TH) domain, with Txk being the only exception. Because PH domains bind phosphoinositides with high affinity, Tec family kinases have been proposed to act downstream of phosphoinositide 3-kinase (PI3K) in signaling pathways. Binding of the PH domain with phosphoinositides is probably required for targeting of Tec family kinases to the cell membrane (2). Tec kinase is activated in response to many upstream signaling events, including antigen receptor, RTK, GPCR, and integrin stimulation (3,4). Activated Tec kinase directly phosphorylates substrates, such as PLC-gamma 2 and BRDG1 docking protein (5), and mediates downstream signaling.

    Alternate Names

    MGC126760; MGC126762; PSCTK4; TEC; tec protein tyrosine kinase; Tyrosine-protein kinase Tec

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