Revision 3
#40142
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, ChIP, C&R
Reactivity:
H M Mk
Sensitivity:
Endogenous
MW (kDa):
300
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q8NFU7
Entrez-Gene Id:
80312
Product Usage Information
For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Chromatin IP | 1:50 |
| CUT&RUN | 1:50 |
Storage
Specificity/Sensitivity
Source / Purification
Background
Background References
- Hermann, A. et al. (2004) Cell Mol Life Sci 61, 2571-87.
- Turek-Plewa, J. and Jagodziński, P.P. (2005) Cell Mol Biol Lett 10, 631-47.
- Okano, M. et al. (1999) Cell 99, 247-57.
- Li, E. et al. (1992) Cell 69, 915-26.
- Tahiliani, M. et al. (2009) Science 324, 930-5.
- He, Y.F. et al. (2011) Science 333, 1303-7.
- Ito, S. et al. (2011) Science 333, 1300-3.
- Ito, S. et al. (2010) Nature 466, 1129-33.
- Shirai, K. et al. (2021) Cancer Sci 112, 2855-2869.
- Bamezai, S. et al. (2021) Leukemia 35, 389-403.
- Good, C.R. et al. (2018) Cancer Res 78, 4126-4137.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting ChIP: Chromatin IP C&R: CUT&RUN
Cross-Reactivity Key
H: Human M: Mouse Mk: Monkey
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
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