WB, IP, IHC-P
H M R Mk
Endogenous
140, 160, 180
Rabbit IgG
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human TIF1α/TRIM24 protein.
Background
TIF1α, also known as TRIM24, is a member of the TIF1 (transcriptional intermediary factor 1) family, a group of transcriptional regulators that play key roles in development and differentiation. Members of this family are characterized by the presence of two conserved motifs – an N-terminal RING-B box-coiled-coil motif and a C-terminal PHD finger and bromodomain unit (1,2). TIF1α binds to the ligand binding domain of several nuclear receptors to regulate their transcriptional activity (2-4). While initially found to attenuate activities of RAR and protect against hepatocellular carcinomas, TIF1α also plays a role in ERα, AR, and VDR signaling (5-9). TIF1α also functions as an E3 ubiquitin ligase, and negatively regulates p53 (10,11). TIF1α is phosphorylated by ATM at Ser768 upon DNA damage, a modification which disrupts TIF1α-p53 interaction (12). TIF1α is overexpressed in numerous cancer types, and acts via numerous oncogenic signaling pathways including PI3K/Akt, Wnt, and AMPK (13-17).
- Le Douarin, B. et al. (1995) EMBO J 14, 2020-33.
- Le Douarin, B. et al. (1996) EMBO J 15, 6701-15.
- Heery, D.M. et al. (1997) Nature 387, 733-6.
- Le Douarin, B. et al. (1998) J Steroid Biochem Mol Biol 65, 43-50.
- Khetchoumian, K. et al. (2008) Cell Cycle 7, 3647-52.
- Ignat, M. et al. (2008) Proc Natl Acad Sci U S A 105, 2598-603.
- Khetchoumian, K. et al. (2007) Nat Genet 39, 1500-6.
- Tsai, W.W. et al. (2010) Nature 468, 927-32.
- Kikuchi, M. et al. (2009) Biochim Biophys Acta 1793, 1828-36.
- Allton, K. et al. (2009) Proc Natl Acad Sci U S A 106, 11612-6.
- Tai, E. and Benchimol, S. (2009) Proc Natl Acad Sci U S A 106, 11431-2.
- Jain, A.K. et al. (2014) Mol Cell Biol 34, 2695-709.
- Li, H. et al. (2012) PLoS One 7, e37657.
- Zhang, L.H. et al. (2015) Oncogene 34, 600-10.
- Fang, Z. et al. (2017) Oncol Lett 13, 1797-1806.
- Lv, D. et al. (2017) Nat Commun 8, 1454.
- Zhu, Y. et al. (2018) Exp Cell Res 367, 274-281.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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