WB, IP
H
Endogenous
18, 23
Rabbit
#Q02223
608
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu115 of human TNFRSF17/BCMA protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
B cell maturation antigen (BCMA/TNFRSF17/CD269) is a transmembrane glycoprotein and member of the TNFR superfamily (1). BCMA expression is largely restricted to the B cell lineage. Pro-survival signaling through this receptor plays a pivotal role in humoral immunity by regulating B cell maturation and plasma cell differentiation upon binding its ligands, BAFF and APRIL (2-6). BCMA is expressed in a number of B cell malignancies and has garnered much attention as a novel therapeutic target for the treatment of multiple myeloma due to its selective and elevated expression on the cell surface of malignant plasma cells (7-10).
- Madry, C. et al. (1998) Int Immunol 10, 1693-702.
- Chiu, A. et al. (2007) Blood 109, 729-39.
- Avery, D.T. et al. (2003) J Clin Invest 112, 286-97.
- O'Connor, B.P. et al. (2004) J Exp Med 199, 91-8.
- Darce, J.R. et al. (2007) J Immunol 178, 5612-22.
- Xu, S. and Lam, K.P. (2001) Mol Cell Biol 21, 4067-74.
- Carpenter, R.O. et al. (2013) Clin Cancer Res 19, 2048-60.
- Tai, Y.T. et al. (2006) Cancer Res 66, 6675-82.
- Moreaux, J. et al. (2004) Blood 103, 3148-57.
- Tai, Y.T. et al. (2014) Blood 123, 3128-38.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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