WB, IP, IF-IC, FC-FP
H
Endogenous
75
Rabbit IgG
#Q96NM4
84969
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:3200 - 1:6400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu343 of human Tox2 protein.
Background
Thymocyte selection-associated high mobility group box protein (Tox) is a DNA-binding nuclear factor and member of the evolutionarily conserved high-motility group (HMG)-box superfamily. Tox also defines a small subfamily of proteins that include Tox2, Tox3, and Tox4, all of which are highly conserved in vertebrate species but have unique tissue expression patterns and functions (1,2).
Tox2 is expressed by natural killer (NK) cells, T follicular helper (Tfh) cells, and exhausted CD8+ T cells (3,4). Tox2 and BCL6 play a critical role in Tfh cell development by generating a transcriptional feed-forward loop that drives the Tfh program (5). Tox2 induction plays a role in driving CD8+ T cell exhaustion and is being investigated as a therapeutic target for cancer immunotherapy (6).
- O'Flaherty, E. and Kaye, J. (2003) BMC Genomics 4, 13.
- Aliahmad, P. et al. (2012) Curr Opin Immunol 24, 173-7.
- Vong, Q.P. et al. (2014) Blood 124, 3905-13.
- Han, J. et al. (2022) Clin Exp Immunol 208, 268-280.
- Xu, W. et al. (2019) Immunity 51, 826-839.e5.
- Seo, H. et al. (2019) Proc Natl Acad Sci USA 116, 12410-12415.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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